【作者】 陈春满； 叶一枝； 凌绪柏；

【Author】 Chen Chunman;Ye Yizhi;Ling Xubo Dongguan Biotechnology Institute Dongguan, Guangdong 523086 Shiwan Agricultural Technology Center Boluo 516125 College of Agronomy South China Tropical Agricultural University Danzhou 571737

【机构】 东莞市生物技术研究所； 广东省博罗县石湾农业技术中心； 华南热带农业大学农学院；

【摘要】 黄花美冠兰休眠芽外植体在MS+3mg/L BA+0.1mg/L NAA的培养基上诱导产生原球茎;原球茎在MS+3～4mg/L BA+0.1～0.2mg/L NAA的培养基上增殖并实现芽的分化;小芽在MS+1.2mg/L IBA+0.2mg/L NAA+100g/L香蕉果肉的培养基上生根,从而形成完整的再生植株。更多还原

【Abstract】 Methods for efficient in vitro propagation of Eulophia flave (Lindl.) Hook.f. aredescribed in this paper Shoot-tips were used as explants and MS was used as the basal mediumfor all the cultures. Protocorm-like bodies (PLBs) were induced on a medium supplemented withNAA 0.1 mg/L and BA 3.0 mg/L, multiplication of the PLBs and differentiation of plantletswere achieved on a medium supplemented with NAA 0.1～0.2 mg/L and BA3～4 mg/L, and theinitiation of roots were realized on MS medium supplemented with NAA 0.2 mg/L, IBA 1.2mg/L and banana juice 10%.更多还原