【作者】 汪俊军； 张春妮； 庄一义；

【Author】 WANG Jun - jun, et al. Center of Laboratory Medicine Science of PLA, Nanjing General Army Hospital, Nanjing 210002, China

【机构】 南京军区南京总医院全军医学检验中心；

【摘要】 目的探讨脂蛋白循环免疫复合物(IC)致动脉粥样硬化作用。方法:分别采用人源的天然、氧化低密度脂蛋白(LDL),脂蛋白(a)与异源的抗载脂蛋白B(apoB)制备IC,并观察了不同IC与巨噬细胞的相互作用。结果:天然、氧化LDL及Lp(a)制备的IC均引起巨噬细胞内胆固醇酯(CE)大量堆积(P<0.001),且随着剂量的增加而升高,并转化为泡沫细胞;而天然LDL、抗apoB处理的巨噬细胞内未见CE的堆积。结论:天然、氧化LDL及脂蛋白(a)循环免疫复合物均导致巨噬细胞转化为泡沫细胞,参与动脉粥样硬化形成。更多还原

【Abstract】 Objective To investigate the atherogenic role of Lipoprotein immune complexes (IC). Methods Nature, oxidized LDL and Lipoprotein (a) [Lp (a)] IC were prepared by using human Lipopro-tein interaction with sheep antibodies against human LDL. And the effect of various lipoprotein IC with different level inducing cholesteryl ester accumulation in human macrophages was studied. Results Nature, oxidized LDL and Lipoprotein (a) [Lp (a) ] IC all induced cholesteryl ester accumulation in human macrophages (P <0. 001) , and the macrophages were found translated into foams. The degree of cholesteryl ester accumulation in macrophages improved, along with IC level increasing. Conclusions Lipoprotein IC translate macrophages into foams and partcipate in atherosclerotic formation.更多还原