【作者】 沈丽佳； 阮萍； 殷操； 谢思明； 赵彤；

【Author】 SHEN Li-Jia,RUAN Ping,YIN Cao,XIE Si-mmg,ZHAO Tong. (Department of Stomatology,Jinan University: Guangzhou 510632,Guangdong Province;Department of Oral Mucosal Diseases, Guangdong Provincial Stomatological Hospital.Guangzhou 510260,Guangdong Province;Department of Pathology,First Military Medical University,Guangzhou 510515,Guangdong Province,China)

【机构】 暨南大学医学院口腔医学系； 广东省口腔医院黏膜病科； 第一军医大学病理学教研室；

【摘要】 目的:探讨口腔扁平苔藓TNF-α、肿瘤坏死因子受体I的表达意义及其与细胞凋亡的关系。方法:采用脱氧核糖核苷酸末端转移酶介导的原位缺口末端标记法(TUNEL法)、免疫组化技术检测50例口腔扁平苔藓及10例正常口腔黏膜中TNF-α、肿瘤坏死因子受体I(TNFRI)的表达及细胞凋亡情况。结果:口腔扁平苔藓的上皮角质细胞FNFR I及固有层TNF-α表达均显著高于正常对照组,而上皮FNF-α及固有层TNFR I的表达则显著少于正常对照组。口腔扁平苔藓的上皮层可见大量凋亡细胞,而固有层单个核细胞的凋亡指数则低于正常对照组。结论:口腔扁平苔藓中同时存在角质细胞的凋亡亢进及单个核细胞凋亡的减少,这种凋亡的异常可能与口腔扁平苔藓的发生发展密切相关。更多还原

【Abstract】 PURPOSE: To examine the expression and distribution of tumor necrosis factor-α(TNF-α)/ tumor necrosis factor receptor I (TNFR I)and apoptosis in oral lichen planus, and evaluate their roles and relation in oral lichen planus. METHODS: Immunohistochemicalassay and TUNEL were employed to study the expression of TNF-α, TNFR I and apoptosis in 50 oral lichen planus cases and 10 normal oral mucosa specimens. RESULTS: Compared with the normal control group, TNF-α expression was with upregulation of mononuclear cells in lamina propria and with downregulation of keratinocytes in oral lichen planus lesion (P<0.05). On the contrary, TNFR I expression was increased in keratinocytes and decreased in lamina propria in oral lichen planus lesion (P<0.05).The increased apoptosis index in keratinocytes and the decreased apoptosis index in lamina propria were found in oral lichen planus (P<0.05). CONCLUSIONS: The accelerated apoptosis of keratinocytes and the inhibition of lymphocytes apoptosis may contribute to the formation and progression of oral lichen planus更多还原