【作者】 巩薇； 卫礼； 王吉； 贺争鸣； 邢瑞昌；

【Author】 GONG Wei,WEI Li,WANG Ji,HE Zhengming,XING Ruichang(National Institute for the Control of Pharmaceutical and Biological Products, National Monitoring Center of Laboratory Animals(Genetics and Microbiology), Beijing 100050)

【机构】 中国药品生物制品检定所；

【摘要】 目的建立小鼠弓形虫抗体检测方法。方法制备抗原片和可溶性抗原,免疫小鼠制备阳性对照血清,确定了IFA和ELISA检测方法的最适工作条件。根据弓形虫B1基因序列,合成一对寡聚脱氧核糖核苷酸片段,建立PCR检测体系。结果与IHA法相比较,ELISA、IFA的抗体检出率均显著高于IHA,抗体滴度分析,ELISA抗体滴度高于IFA法。对弓形虫感染鼠不同组织进行PCR检测,可检出特异性扩增条带。结论建立了IFA和ELISA血清学检测方法,具有灵敏度高、重复性好,结果判定明确等优点,是比较有效的监测方法。建立了弓形虫PCR检测体系,经对弓形虫DNA及病鼠各组织的初步检测,证明本PCR体系具有较高的敏感性和特异性。更多还原

【Abstract】 Objective To establish the method for detecting mouse Toxoplasmosis. Methods The antigen and positive serum of Toxoplasmosis were prepared from the mice. Two pairs of primers were designed and synthesized based on the sequence of Toxoplasma gondii Bl, the were used the PCR. Results The positive rate detected by IFA and ELISA was significantly higher than that by indirect haemagglutination (IHA) and antibody litres in ELISA was higher than in IFA. Conclusion The IFA and ELISA method can detect mouse Toxoplasmosis with high sensitivity. the experiments this study also showed that the PCR and detect Toxoplasma gondii DNA and diagnose toxolpasmosis with high specificity and sensitivity.更多还原