【作者】 金万梅； 巩振辉； 吕元红；

【Author】 Jin Wanmei, Gong Zhenhui and Lu Yuanhong(College of Horticulture, Northwest Sci-Tech University of Agriculture and Forestry, Yangling Shaanxi 712100,China;Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Science, Beijing 100093,China)

【机构】 西北农林科技大学园艺学院；

【摘要】 以蕾期的芥菜(Brassica juncea Coss)生态型雪里蕻和圆叶芥为试材,在携带潮霉素磷酸转移酶标记基因的根癌农杆菌(Agrbacterium tumefaciens)菌株GV3101的介导下,对影响芥菜植株原位真空渗入遗传转化效果的真空渗入时间、根癌农杆菌悬浮孢子的浓度、渗入培养基、感染方式等进行研究。结果表明,真空渗入时间为2 min/间歇30s/30s;菌液浓度OD600为0.8;渗入培养基为1/2MS无机+B5有机;菌株采用强感染型的;对花蕾进行真空渗入,可以获得最佳转化效果。更多还原

【Abstract】 In bud stage of mustards (Brassica juncea Coss)ecotype Xuelihong and Yun-yejie were transformed with in planta vacuum infiltration by an Agrobacterium tumefaciens strain GV3101 carrying hygromycin resistance gene. Effects of different factors on transformation frequency with in planta vacuum infiltration were studied. The best transformation frequency was obtained in the following conditions, the vacuum infiltration time was 2 min/ intermission 30s/30s, the concentration of Agrobacterium cells was OD6000. 8, infiltration medium was 1/2MS inorganic composition and B5 organic composition, and in bud stage the buds were treated by the vacuum infiltration.更多还原