【作者】 张明方； 于天祥； 毛碧增； 何祖华；

【Author】 Zhang Mingfang Yu Tianxiang Mao Bizeng He Zhuhua(College of Agriculture and Biothechnology,Zhejiang University,Hangzhou 310029,China;National Laboratory of Plant Molecular Genetics,Shanghai Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China)

【机构】 浙江大学农业与生物技术学院； 中国科学院上海生命科学院植物生理生态研究所；

【摘要】 采用根癌农杆菌(Agrobacterium tumefaciens)介导法将葡聚糖酶和几丁质酶基因共同导入西瓜(Citrullus lanatua)子叶外植体,经抗性筛选和组织培养获得再生植株。通过对转基因植株中插入序列的GUS染色鉴定和特异引物PCR检测。表明葡聚糖酶和几丁质酶基因已成功导入西瓜植株.更多还原

【Abstract】 A construct containing a rice chitinase gene and an alfalfa glucanase gene was co-transferred into watermelon through Agrobacterium tumefaciens-mediated method. Plantlets were regenerated in vitro by resistance selection on MS medium containing various concentions of kanamycin. GUS and PCR-aided assays indicated that both chitinase and glucanase genes have been co-transferred into watermelon successfully.更多还原