【作者】 郭军； 屈冬玉； 王晓武； 金黎平； 谢开云； Rays Jiang； Francine Govers；

【Author】 Guo Jun,Qu Dongyu,Wang Xiaowu,Jin Liping,Xie Kaiyun,Jiang Rays,Govers Francine(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Laboratory of Phytopathology,Wageningen University and Graduate School of Experimental Plant Sciences,Binnenhaven 9,6709 PD Wageningen,The Netherlands)

【机构】 中国农科院蔬菜花卉研究所； Laboratory of Phytopathology,Wageningen University and Graduate School of Experimental Plant Sciences,Binnenhaven 9,6709 PD Wageningen,The Netherlands；

【摘要】 通过对由具有6个无毒基因不同表现型的马铃薯晚疫病菌构建的4个混合池进行cDNA-AFLP分析,共获得85个与无毒基因相关的差异表达片段,其中与无毒基因Avr1、Avr2、Avr3-Avr10-Avr11、Avr4相关的差异表达片段分别为20, 28, 16和21个。将这些差异表达片段对20个晚疫病病菌个体进行无毒基因差异表达验证,得到无毒基因连锁群Avr3-Avr10-Avr11的候选片段1个,即A11T13Avr3-10-11S157;无毒基因Avr4的候选片段2个,即A24T19Avr4S125和A24T24Avr4S144。Blast分析表明:A11T13Avr3-10-11S157与马铃薯晚疫病菌正萌发孢子囊(germinating sporangium)EST(expressed sequence tag)库中的序列PG001F10.XT7同源;A24T19Avr4S125与晚疫病菌正萌发孢囊(germinating cyst)EST库中的PH051G10.XT7部分序列完全一致;未发现与A24T24Avr4S144同源的序列,说明此序列可能为一新的马铃薯晚疫病菌EST.A24T19Avr4S125与EST PH051G10.XT7的序列一致性表明选择正在萌发的孢囊时期鉴定无毒基因的可行性,也为进一步快速获得全长无毒基因Avr4及进行功能验证奠定了基础.更多还原

【Abstract】 Through the cDNA-AFLP analysis of 4 pools constructed by different phenotypes of 6 avirulence genes in Phytophthora infestans,total 85 candidate transcript derived fragments (TDFs) were obtained,which were 20 for Awl,28 for Avr2,16 for Avr3-Avr10-Avr11,21 for Avr4,respectively. One candidate TDF of linkage genes Avr3-Avr10-Avr11(A11T13Avr3-10-11S157) and two candidate TDFs of Avr4 (A24T19Avr4S125 and A24T24AvrS144) were preliminarily acquired by verification of these TDFs with 20 P. infestans individuals. The results of Blast analysis showed that the A11T13Avr3-10-11S157 was homologous with EST G001F10.XT7,and the A2AT19Avr4S125 had the same sequence with EST PH051G10.XT7 in Phytophthora germinating cyst EST library . No homologous sequence wan found with A24T24Avr4S144,it is indicated that this TDF could be a new EST of P. infestans. The fact of A24T194vr4S125 with the same sequence of EST PH051G10.XT7 demonstrated that selecting germinating cyst for identification of avirulence genes of P. infestans is available,and it could settle the base for quickly obtaining full length and the function analysis of Avr4 gene in the future.更多还原