【作者】 孙志栋； 陈惠云；

【Author】 Sun Zhi-dong Chen Hui-yun(Ningbo Academy of Agriculture and Sciences,Ningbo 315040,China,)

【机构】 宁波市农科院；

【摘要】 SDS法和CTAB法是提取植物基因组DNA的两种基本方法。本文以多酚类植物棉花为研究材料,对SDS法、CTAB法、改良SDS法和改良CTAB法进行了比较研究,结果表明:改进的SDS法和改进的CTAB法分别优于原SDS法和原CTAB法,两种改进的方法对提取棉花基因组DNA有异曲同工之妙。DNA的得率不仅与提取方法有关,而且与棉花取材的部位不同有关。改进的CTAB法DNA得率高于改进的SDS法:棉花不同部位材料采用改进的CTAB法提取基因组DNA,其DNA得率的高低依次为花药>花瓣>叶片>纤维。由改进方法提取的棉花基因组DNA能完全双酶切.满足SSR和AFLP等后续分子生物学研究的需要。而且两种改进的方法也适应于其它植物基因组DNA的提取,其中改进的SDS法尤适于高蛋白、粘多糖类植物基因组DNA的提取,而改进的CTAB法则适于多酚类植物基因组DNA的提取。更多还原

【Abstract】 The four methods of isolating genomic DNA from polyphenolic plant were compared with each other in this paper. The results showed that both the modified SDS and CTAB methods are better than the original SDS and CTAB methods. Yield of genomic DNA was not only related to extraction methods,but also to cotton tissues. DNA yield of modified CTAB method was higher than modified SDS method. Among the material of cotton studied,the yield of DNA was recorded highest from anther followed by petal,leave and fiber. The cotton genomic DNA isolated using modified methods could be digested by enzyme and also good to meet the demand of subsequent molecular operations such as SSR and AFLP analysis. Moreover,the two modified methods were also suitable for plant with high protein or glutinous amylose and the modified for plant with poly-hydroxybenzene.更多还原