【作者】 张祖新； 唐万虎； 邹锡玲； 郑用琏；

【Author】 ZHANG Zu-xin~(1,2);TANG Wan-hu~1;ZOU Xi-ling~1;ZHENG Yong-lian~1 (1 National Key Lab. of Crop Genetic and Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070; 2 College of Life, Yangtze University, Jingzhou, Hubei 434025)

【机构】 华中农业大学作物遗传改良国家重点实验室；

【摘要】 玉米幼苗受淹水胁迫导致根系缺氧。研究具有不同耐渍能力的玉米自交系对淹水胁迫早期响应基因的表达,对于发掘耐渍相关基因、阐述耐渍性的分子机理具有重要意义。本研究采用SSH和cDNA microarray技术,以具有耐渍性差异的玉米自交系Mo17和Hz32为材料,通过比较Mo17和Hz32在淹水早期(0-4h)的基因表达,结果发现,许多参与糖酵解、能量代谢、脂代谢、信号传导、DNA转录、蛋白质的合成与降解、细胞结构以及光合作用等复杂的代谢、结构和生理过程的基因,受淹水处理诱导其表达改变,差异表达的基因表现为6中不同表达模式,并且淹水处理1h与2-4h后的基因表达具有明显的差异,可将根系对淹水处理的响应分为1h前和2h后2个不同阶段。Mo17和Hz32对淹水胁迫的基因表达响应不同,在有氧代谢向厌氧代谢转变以及其他多种代谢和生理转变上,Hz32比Mo17响应更快。基于Mo17和Hz32对淹水胁迫响应基因的表达差异,1个可能的转录因子基因Zm-bRLZ和1个参与甾醇代谢的Cytochrome P450基因家族成员Sicyp51被克隆,并对其可能的功能进行了讨论。更多还原

【Abstract】 Submergence stress result in oxygen limitation in maize roots. The study on waterlogging responsive genes expressed in inbred, which are different tolerance-submergence, is propitious to discover genes associated with submergence tolerance and to elucidate the molecular mechanism of submergence tolerance. In the study, Mo17 and Hz32, two inbred with different tolerance, were used to reveal difference on early responsive genes in roots cell under submerging condition. The data from SSH (suppression subtracted hybridization) and cDNA microarray showed that a few of gene expressions were altered because of inducement of submergence. These genes involve in multiple metabolic, structural and physiological processes, such as glycosis, energy metabolism, lipid metabolism, signal conduction, DNA transcription, protein biosynthesis and digest, cell component and photosynthesis, and displayed six expression patterns. The expressed genes after 1h long submerging treatment were markedly different from that after 2-4h long treatment, the response of roots to submergence stress could be divided into two phases: in 1h and after 2h long submerging treatment. In addition, responses on gene expressions to submergence were also different between Mo17 and Hz32. The conversion from aerobic metabolism to anaerobic metabolism and other metabolic and physiological conversion were faster in Hz32 than that in Mo17. Based on the different responsive pattern of genes in two studied inbred, Zm-bRLZ which encodes putative transcription factor, and Sicyp51 which is a member of Cytochrome P450 family and functions in sterol metabolism, were cloned, their functions were discussed.更多还原