【作者】 宋芸； 夏艳莉； 魏昕； 张志明； 赵茂俊； 潘光堂；

【Author】 SONG Yun1,2;XIA Yan Li2;WEI Xing2;ZHANG Zhi Ming2;ZHAO Mao Jun2;PAN Guang Tang2 (1Maize Research Institute, Sichuan Agricultural University, Ya, an, Sichuan 625014, China 2The College Of Life Science, Shanxi Agricultural University, Tai, gu, Shanxi 030801, China)

【机构】 四川农业大学玉米研究所；

【摘要】 选用玉米高幼胚培养能力自交系18-588(红)与低培养能力自交系R15配制6个世代(P1、P2、F1、B1、B2、F2),并对其进行幼胚培养,测定胚性愈伤组织诱导率、非胚性愈伤组织出愈率、愈伤组织克隆力和愈伤组织绿苗再分化数4个性状作为评价指标,采用6世代均数法、遗传方差分析法及主基因+多基因混合遗传模型6世代联合分析法进行遗传分析,结果表明,在世代均数分析法中,胚愈率及非胚愈率符合加性-显性-上位性基因效应模型,克隆力及再分化数符合加性-显性基因效应模型;利用世代联合分析法,得到胚愈率、非胚愈率、克隆力的最适模型为2对主基因+多基因混合遗传模型,再分化数的最适模型为1对主基因+多基因混合遗传模型。两种方法的分析结果相比发现,世代联合分析法不仅提高了试验结果的精确度,同时还提供了更多的遗传信息。更多还原

【Abstract】 Two kinds of the inbred-line R18-599（hong） with very excellent Embryo Culturing Capacity and R15 with very bad Embryo Culturing Capacity were selected as P₁ and P₂ for obtaining P₁、P₂、F₁、F₂、B₁、B₂ generations. By culturing immature embryo of the six generation, Four culturing characters, which are efficiency of embryonic callus induction、efficiency of non-embryonic callus induction、embryonic callus clone ability and number of regenerating plant, were analyzed with the combing quantitative-character genetic analyzing methods、generations mean analysis、major gene and polygeng model of generation joint analysis. The results of generation mean analysis showed that the embryonic callus induction efficiency and the non-embryonic callus induction efficiency are accorded with additive-domiance-epistatic gene model. The embryo genetic callus clone ability and the number of regenerating plant are accorded with additive-dominance genetic model. The results of generation joint analysis showed that the embryonic callus induction efficiency、the non-embryonic callus induction efficiency and embryo genic callus clone ability are all accorded with two major genes plus polygene mixed inheritance model. The number of regeneration plant is accorded with one major gene plus polygene mixed inheritance model. By the comparison between the two analysis methods, the generation joint analysis may not only raise experimental measure of precision, but also provide more genetic information.更多还原