【作者】 曲丽娜； 杨唐斌； 钟平； 袁艳红； 赵海；

【Author】 Qu Li-na, Yang Tang-bin, Zhong ping, Yuan Yan-hong, Zhao Hai 13th department, Institute of Space Medico-Engineering, Beijing 100094, China

【机构】 航天医学工程研究所；

【摘要】 氧化应激参与许多疾病的病理发生和发展过程,模拟失重和航天飞行增加机体氧化应激水平,影响航天员的健康和工作效率。但迄今为止,有关失重条件下自由基对神经细胞生物大分子的损伤研究资料甚少,因此有必要开展此项研究,并为采取相应的医学保护措施提供参考。氧化损伤的细胞和生物化学表现为膜流动性下降和膜渗透性增加、蛋白氨基酸侧链修饰引起蛋白质(酶)结构和功能的改变,相应的损伤生物标记物有脂质过氧化物、蛋白羰基化产物和硝基酪氨酸等。本研究运用单克隆抗体技术制备了蛋白损伤生物标记物的特异性单克隆抗体,通过DNP和硝基酪氨酸的ELISA和免疫组化检测方法,研究了分化神经样细胞——PC12细胞在回转条件下的氧化应激水平的影响。结果表明回转增加PC12细胞的成活率和数目,降低细胞的膜流动性;褪黑素和槲皮素可降低水溶性和脂溶性脂质过氧化物浓度、降低硝基酪氨酸和羰基化蛋白水平,增加细胞的膜流动性,并提高细胞总的抗氧化能力。本研究从细胞和分子水平评估细胞氧化应激水平,证实褪黑素和槲皮素可对抗模拟微重力对神经细胞的氧化损伤。更多还原

【Abstract】 Evidence suggests that oxidative stress may be increased with spaceflight, and it may be harmful to the health of astronauts and influences their working efficiency. A variety of studies have been performed to examine the effect of space flight and micrograviry on the nervous system, but so far little attention has been focused on the effect of free radicals on nervous system in the microgravity environment. In the present study, we prepared anti-nitrotyrosine and anti-DNP monoclonal antibodies and developed nitrotyrosine and carbonyl protein ELISA for determination of protein oxidative modification. The oxidative stress levels of neuron-like rat pheochromocytoma PC12 cells were assessed systematically on the molecular level using immunological and other detection methods, and the protective effects of antioxidants (melatonin and quercetin) on the neuronal cells were also assessed during the simulated microgravity. The results showed that clinorotation resulted in high PC 12 cell viability. Protein nitration and lipid peroxidation were increased, and total antioxidant capacity was decreased during clinorotation, which confirmed that microgravity could result in increased oxidative stress. Melatonin and quercetin could effectively protect neuronal cells from oxidative damage in the simulated microgravity.更多还原