【作者】 潘爱虎； 张大兵； 潘良文； 陈家华； 袁政； 梁婉琪；

【Author】 Pan Ai-hu;Zhang Da-bing;Pan Liang-wen;Chen Jia-hua;Yuan Zheng;Liang Wan-qi Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agri -biotechResearch Center, Shanghai Academy of Agricultural Sciences, Shanghai 201106 Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135 Horticulture department of JiangXi Agricultural University, NanChang 330045

【机构】 上海市农业科学院生物技术中心上海市农业遗传育种重点实验室； 上海出入境检验检疫局； 江西农业大学园艺系；

【摘要】 随着全球对转基因生物的日益关注,许多国家推出转基因产品标签制度。2001年5月,中国政府公布并实施了转基因农产品标识的规定。为配合我国转基因农产品标签制度的实行,有些实验室开展了转基因产品的检测技术研究。本文报道了利用定性PCR技术检测孟山都公司培育的转基因抗草甘膦油菜中的epsps、gox和fmv35s的方法,同时设立了阳性内对照napin,该方法的检测灵敏度为0.05%;利用复合PCR可以在一个PCR反应中同时检测出epsps、gox、fmv35s和napin基因。这种方法可以有效地用于转基因抗草甘膦油菜中的外源基因的检测。更多还原

【Abstract】 Many countries have introduced the GMO labeling provisions in biosafetyregulation due to the worldwide concern about the safety of genetically modified organisms(GMO). Since the May of 2001, GMO derived products must be labeled in China. Thishas led some official laboratories to develop practical analytical methods that can assist inthe enforcement of the labeling provisions. Here, we reported an efficient polymerasechain reaction method developed for detecting transgenic glyphosate resistant rape varietyproduced by Monsanto Company. The r-DNA-such as epsps, gox and fmv35s promotercould be clearly detected from samples containing above 0.05% glyphosate resistant rapeDNA using this method. A multiplex PCR method was also developed to detect thesegenes in one reaction simultaneously. Thus, this method should be applied in screeningthe extrinsic genes in transgenic glyphosate resistant rape.更多还原