【作者】 刘德璞； 袁鹰； 郑培和； 温刚； 徐文静； 周正平； 高月波；

【Author】 Depu Liu;Ying Yuan;Peihe Zheng;Zhengping Zhou;Wenjing Xu;Gang Wen;Yuebo Gao Jilin Academy of Agricultural Sciences, Gongzhuling,Jilin, 136100

【机构】 吉林省农科院生物技术中心；

【摘要】 对大豆花粉管通道技术进行深入研究。做多种重组基因的转化,获得可重复性结果,确立了此技术在转基因上的应用价值和可行性;建立了转化操作、筛选鉴定的技术;转化了抗大豆蚜虫的GNA基因和抗食心虫的Bt.基因,还成功转移了Bar、Gus、NPTH、Barnase、反义ACC等基因,对转基因大豆进行了抗虫性鉴定和分子生物学鉴定;利用此技术培育出经农业部转基因安全管理部门批准释放的转GNA抗蚜大豆的品系和通过转总DNA而获得的两个已审定品种。分子生物学鉴定结果及育种效果证明大豆花粉管通道技术是成功的、有效的、科学的。更多还原

【Abstract】 Research on soybean pollen tube pathway was carried out.GNA Genewas induced into Jilin30 and Jilin20 and Bt gene was induced into jilin 27 and D3053through pollen tube pathway technique and transgenic plants were gained. Molecularbiologica1 evidence results showed that they were transgenic. The resistant lines to aphiswere gained by evaluating and screening in field. Bar, Gus, NPTII, Barnase gene werealso induced into soybean by this method. The results of transformation and breedingproved that pollen tube pathway technique was useful, successful in soybean genetransformation.更多还原