【作者】 邹瑜； 林贵美； 张瑛； 牟海飞； 何海旺； 韦华芳；

【Author】 Zou Yu,Lin Gui-mei,Zhang Ying,Mou Hai-fei,He Hai-wang, Wei hua-fang (Guangxi Plant Tissue culture CO.Ltd, Guangxi Academy of Agricultural Sciences,Nanning,530007)

【机构】 广西农科院植物组培苗有限公司；

【摘要】 毛葡萄组织培养,通常存在玻璃化和成本高、成活事低等问题,不利于产业化生产,从技术路线入手,探索毛葡萄产业化育苗新工艺,经多年研究及生产应用表明:在1/2MS+BA0.01—0.05+IAA1.0—2.0培养基上,培养光照强度1500—2000lux,温度27-30℃,采用组培微型扦插方式,可以实现毛葡萄组培育苗产业化。以塘泥:椰糠或河沙=2:1为基质,假植成活事可达90%以上。更多还原

【Abstract】 Because of vitrification, higher cost and lower survival rate in traditionally tissue culture of Vitis quinquangularis Rehd., it is very disadvantage to commercial application. So we summary a conclusion that new propagating technology of Vitis quinquangularis Rehd. from difference technique route of tissue culture.studied and produce applied by many years, the result showed that we can realize commercial application of clone’s propagating technology of Vitis quinquangularis Rehd. by way of microcuttage in the condition of 1/2MS+BA0.01-0.05+IAA1.0-2.0,1500-2000lux,27-30℃. The clone’s survival rate of tranfer can attain more than 90% in the combination of media of two pieces of spond studge and a pieces of conconut cake of sade.更多还原