【作者】 谢志勤； 谢芝勋； 邓显文； 庞耀珊； 廖敏； 刘加波； 唐小飞； 何竞铭；

【Author】 Xie Zhiqin, Xie Zhixun, Deng Xianwen, Pang Yaoshang,Liao min, Liu Jiabo , Tang Xiaofei, He Jingming(Guangxi Veterinary Research Institute, Nanning 530001)

【机构】 广西兽医研究所；

【摘要】 本文报告了采用随机扩增多态性DNA(RAPD)技术.以随机引物成对组合的方式.对鸡毒支原体(MG)5个广西分离株和3个标准株DNA进行了.多态性分析.结果显示,所选用的20条引物中.有3对(6条)引物扩增的条带在2-10条之间,大小在200-3000bp之间。指数分析显示.MG分离株Y2与WL、CH与Y3的相似指数最高.分别为0.92和0.86.与另一MG分离株H2的相似指数在0.36-0.62之间。3抹MG标准毒株S6、K2221、K1501与分离株间的相似指数在0.17-0.57之间。表明广西流行的鸡毒支原体与标准株存在差异.流行的毒株也呈现多样性。更多还原

【Abstract】 The polymophism of MG 3 reference strains and 5 Guangxi isolates were analysied by RAPD using the combination of arbitrary primers. The amplification result shows that 2-10 bands were amplified with 6 out of 20 primers, and the length of the bands was between 200 bp to 3000 bp. The result shows the similar index of isolate Y2 strain and WL strain is 0. 92, CH strain and Y3 strain is 0. 86, the 4 isolated strains Y2, WL, CH, Y3 and H2 is between 0. 36-0. 62, 3 reference strains S6, K2221, K1501 and Guangxi isolates is between 0. 17 -0. 57. The results indicate that MG isolates of Guangxi are showing polymophism, and different from the refrence strains.更多还原