【作者】 王邠； 肖新霞； 邓伟峰； 梁鸿； 赵玉英；

【Author】 WANG Bin, XIAO Xin-xia, DENG Wei-feng, LIANG Hong, ZHAO Yu-yingDepartment of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100083;Huaihua Medical College of Hunan, Huaihua 418000

【机构】 北京大学药学院天然药物学系； 湖南省怀化医学高等专科学校；

【摘要】 应用高效液相色谱-紫外检测器联用法测定酸枣仁中酸枣仁皂苷A及B含量,采用RP-C₁₈色谱柱,CH₃CN:0.1％H₃PO₄:H₂O（36:30:34）为流动相,流速1ml/min,204nm为检测波长,温度为室温。分析测定了9种产地酸枣仁商品药材中酸枣仁皂苷A和B的含量。酸枣仁皂苷A及B在分别3.24-9.72μg和2.48-7.44μg范围内面积与其进样量线性关系良好（r_A=0.99980;r_B=0.99998）;样品平均回收率分别为99.81％（RSD=3.31％）和94.62％（RSD=2.03％）。该方法快速简便,灵敏度高,重现性好,易于普及,可用于酸枣仁药材的质量控制。更多还原

【Abstract】 High performance liquid chromatography with ultro violet detector (HPLC-UV) was used to determine the content of jujubosides A and B. The HPLC was performed on a reversed phase C18 column by using CH3CN:0.1%H3PO4:H2O (36:30:34) as mobile phase at a flow rate of 1ml/min, the UV detection wavelength was 204nm and column temperature was room temperature. Nine samples were determined. The calibration curves were linear (rA=0.99980: rB=0.99998) in the range of 3. 24-9. 72μg and 2. 48-7. 44μg and the mean recovery was 99. 81% (RSD=3. 31%) and 94.62% (RSD=2.03%) for jujubos’ide A and jujuboside B, respectively. This method is simple, rapid, sensitive and reliable.更多还原