【作者】 马保亮； 范翊； 张立功； 孔祥贵； 李亚军；

【Author】 MA Bao-liang;FAN Yi;ZHANG Li-gong;KONG Xiang-gui;LI Ya-jun Changchun Institute of Optic Fine Mechanics and Physics, Chinese Academy of Science, Changchun, 130021, China

【机构】 中国科学院长春光学精密机械与物理研究所；

【摘要】 带有甘露糖的聚双炔薄膜能够识别大肠杆菌并与它们结合,更有趣的是结合可以导致聚双炔薄膜的颜色发生改变,这种颜色变化很容易用裸眼观察到,并且可以通过可见吸收光谱定量分析。这种通过聚双炔薄膜对分子识别的直接检测方法不仅为该薄膜在生物传感器发展领域中的应用开辟了一条新途径,而且为诊断应用和筛选新的结合配体提供了可能。此外,为了理解颜色变化的机理,我们用共振拉曼光谱和付立叶交换红外光谱对聚双炔的亲合变色特性进行了检查。结果表明:当由蓝到红的颜色变化发生时,聚合物骨架的侧链进行了重新排列,同时聚合物骨架的电子结构由炔的形式转变为三烯的形式.更多还原

【Abstract】 The membranes of polydiacetylene backbone decorated with mannose can recognize Escherichia coli (E.coli)and bind with them. The binding leads to the color transition of the membranes, which is readily visible to the naked eyesand can be quantified by visible absorption spectroscopy The direct colorimetric detection by polydiacetylenemembranes not only opens a new path for the use of these membranes in the area of biosensor development but alsooffers new possibilities for diagnostic applications and screening for binding ligand. To understand the mechanism of thechromatic transition, the affinochromism properties of polydiacetylene are examined by resonance Raman (RR) andFourier transform infrared (FTIR) spectroscopy.The results demonstrate the side chains of polymer backbone performrearrangement and the electronic structure in the polymer backbone changes from a acetylene to butatriene form whenthe blue to red chromatic transformation occurs.更多还原