【作者】 杨戎； 刘孝云； 魏莎莉；

【Author】 Yang Rong Liu Xiaoyun Wei Shali(Reproductive and Physical Laboratory,Chongqing University of Medical Sciences,Chongqing 400016)

【机构】 重庆医科大学生殖生理教研室；

【摘要】 以精子前向运动率（PMS）、活动精子百分率（MS）、精子尾部低渗肿胀率（HOS）和精子DNA荧光染色有效精子计数（ESC）为观察指标,探讨中重度子宫内膜异位症（EM）并伴不育患者的腹腔液（PF）对上述几项精子参数的影响。结果发现,中重度EM患者PF与未经处理的精液混合孵育8 h可明显降低PMS,MS和HOS.孵育24 h后,这种抑制作用进一步增强;而经洗涤后的精子与PF混合孵育 8 h后对上述几项参数没有明显影响,但孵育24 h后PMS和MS有明显降低;ESC在上述两种情况中均未见到明显影响.结果提示,中重度EM患者PF对PMS,MS和HOS有明显的抑制作用,而精浆中的某些细胞成分在PF抑制精子活性及损伤膜的完整性中可能起着中介作用,这可能是EM致不育的原因之一。更多还原

【Abstract】 The effect of peritoneal fluid (PF) from patients with endometriosis on human sperm motility parameters were studied. The result showed that progressive motile spermatozoa (PMS),motile spermatozoa (MS) and hypo-osmotic swelling (HOS) of washing-free sperm were significantly inhibited by PF from patients with endometrissis after the 8-hour incubation, and the inhibiting effect was further increased after the 24-hour incubation. PMS, MS and HOS of washing sperm were not significantly decreased by the PF after the 8-hourincubation,but PMS and MS were significantly decreased after the 24-hour incubation. We suggested that PF from patients with moderate and severe endometriosis might inhibit sperm motility or velocity,thus cause infertility.更多还原