【作者】 施用晖； 乐国伟； 方静； 周颐； 王国兴；

【Author】 Shi Yonghui Le Guowei Fang Jing Zhou Yi Wang Guoxing(Food College of Wuxi University of Light Industry Wuxi 214036)

【机构】 无锡轻工大学饲料研究所； 四川农业大学；

【摘要】 试验用4～11日龄艾维茵母系公雏,按理想氨基酸模式,用蛋清蛋白与游离氨基酸配制完整蛋白质比例不同的3种饲粮,完整蛋白质的氨基酸占总氨基酸比例为88.6%(第一组)、44.3%(第二组)、0%(第三组),探讨不同比例完整蛋白质饲粮对肉雏鸡肠道细胞形态结构的影响。光学显微镜观测结果为:含有完整蛋白组十二指肠、空肠上段绒毛高度显著、极显著地(P<0.05～0.01)高于游离氨基酸组。腺窝深度在第一、二组十二指肠、第一组空肠上段极显著地深于(P<0.01)第三组;第二、三组绒腺比高于第一组。透射电镜观察,十二指肠、空肠微绒毛高度均随蛋白质比例而显著增加(P<0.01);游离氨基酸组十二指肠、空肠吸收细胞线粒体、滑面内质网发达,线粒体较长。以长和径比计算体积大小,十二指肠依次为第一、二和三组,3组间差异极显著(P<0.01);空肠依次为第一,三和二组。实验证实,饲粮完整蛋白质及游离氨基酸比例影响肉雏鸡小肠黏膜细胞的形态结构。更多还原

【Abstract】 The effect of dietary intact protein ratio on the morphometric and ultrastructural changes in jejunum and ileum mucosa of broilers were investigated. Broilers were fed three difference diet which percentage of albumen to total amino acid amounts were 88.6%(group 1), 44.3%(group 2) and 0% (group 3), respectively. The villous heightened and crypt deepened in jejunum and ileum significantly in group 1 and 2. The villous height: crypt depth ratio were higher in group 2 and 3 than that in group 1. Electronic micrographs showed that microvilli height were reduced with amino acid diet and an increase in the smooth endoplasmic reticulum and mitochondria were also found in group 3. The result showed that the morphological changes in intestinal mucosa of broilers were affected by different dietary intact protein ratio.更多还原