【作者】 王永坤； 严维巍； 周继宏； 田慧芳； 朱国强； 钱忠明； 李玉峰； 刘华雷； 庄国宏； 王建业； 郁斌；

【Author】 Wang-Yongkun Yan-Weiwei Zhou-Jihong Tian-Huifang et al(College of Animal science and Veterinary Medicine Yangzhou University,Yangzhou, Jiangsu 225009, P. R. of China)

【机构】 扬州大学畜牧兽医学院动医系；

【摘要】 用SPF鸡胚分别从不同患病鸡群分离到4株病毒,经病毒形态、结构、理化和生物学特性、血清学等研究,本病毒为禽副粘病毒Ⅰ型;根据国际上规定三项判定新城疫毒力的标准,4株病毒均具有与新城疫病毒速发型相似的毒力,属于强毒力的毒株;人工感染71周龄SPF鸡均100%发病致死;应用一株鹅副粘病毒制备的单克隆抗体,获得具有对禽副粘病毒Ⅰ型共同的单抗和仅对鹅副粘病毒毒株和鸡副粘病毒毒株呈阳性反应,而对新城疫F48E8、LaSota等毒株不发生反应的特异性单克隆抗体;应用RT-PCR技术对禽副粘病毒Ⅰ型分离株F基因的部分片段进行扩增、测序,并绘制基因进化树作分析,结果表明鸡副粘病毒毒株为基因Ⅶ型。应用Y98毒株和T99毒株制备的油乳剂灭活苗经试验和生产应用表明有良好的保护率。更多还原

【Abstract】 Four virus isolates were obtained from different diseased chicken flocks using SPF chicken embryos. All were identified as avian Paramyxovirus type 1 by morphological examination, biological property, and serological tests. These four isolates all were confirmed to be velo-genic Newcastle disease virus (NDV) based on mean death time (MDT) of chicken embryos, in-tracerebral pathogenicity index (ICPI) in 1-day-old chicken, and intravenous pathogenicity index (IVPI) in 6-week-old chicken. 100% of 71-week-old SPF chickens showed dead after artificial infection. Different mAbs were developed from a Goose paramyxovirus: mAbs react with all avian paramyxovirus type 1; mAbs react only with Goose paramyxovirus and chicken paramyxovirus isolates but not with NDV strain F48 and LaSota. Parts of the fusion gene of the chicken Paramyxovirus isolates were amplified by RT-PCR and sequenced. A phylogenetic tree base on obtained sequences of reference NDV strains were constructed and showed that the isolate was similar to the genotype Ⅶ NDV virus, a novel genotype NDV. The inactivated vaccine made from isolate Y98 and isolate T99 could give good protection against infection.更多还原