【作者】 孟松树； 张绍杰； 郭鑫； 童光志；

【Author】 MENG Songshu, ZHANG Shaojie,GUO Xin,TONG Guangzhi(National Key Laboratory of Veterinary Biotechnology Harbin VeterinaryResearch Institute of CAAS, Harbin 150001)

【机构】 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室；

【摘要】 本研究探讨了ILTV基因免疫所诱导的细胞与体液免疫应答的动态变化。以表达ILTV王岗株gB、gC、gD的真核表达质粒单独或联合免疫SPF鸡,以MTT法检测鸡胸腺、法氏囊淋巴细胞经丝分裂原ConA、PMA刺激后的增殖反应活性,以间接ELISA法检测血清抗体水平。结果表明,ILTV基因免疫诱导机体产生了较强的淋巴细胞增殖反应,血清抗体在免疫后第2周发生阳转,而对照组无相应变化。这是ILTV基因免疫研究的首次报道。更多还原

【Abstract】 Cellular and humoral responses to ILTV DNA vaccine were detected. SPF chickens were immunized with ILTV DNA vaccines (PgB or PgB+PgC+PgD). The proliferative responses of lymphocytes from thymus and bursa of chickens were measured by MTT assay, antibody responses were assayed using indirect ELISA. The results showed that ILTV DNA vaccines induced strong proliferative responses of lymphocytes, seroconversion was observed by two weeks after first immunization, antibody responses peaked after second immunization while the control groups showed no changes. Gene vaccination against ILTV was first reported in this research.更多还原