【作者】 曹殿军； 郭鑫； 梁荣； 闫丽辉； 刘培欣； 闵平； 陈杰；

【Author】 Cao Dianjun, Guo Xin, Liang Rong, Yan Lihui, Liu Peixin , Min Ping, Chen Jie (National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of CAAS, Harbin 150001)

【机构】 中国农科院哈尔滨兽医研究所兽医生物技术国家重点实验室；

【摘要】 本研究根据新城疫病毒(NDV)F基因编码区1-374位核苷酸序列计算其遗传距离并绘制了NDV的系统发育进化树,将68株NDV分为九个基因型(30株为国内分离株),其中Ⅰ～Ⅵ是早已存在的老基因型,Ⅶ、Ⅷ、Ⅸ为新发现的基因型,特别是Ⅸ为我国特有的基因型(F48E9、M3、HLJ-3、HeB-1P和NM-5)。1997～1999年在我国云南、广西、甘肃、陕西、新疆等地分离的YN-1P、GX-3、H1、H2、P1、GX-1、GX-2、GS-2、GS-3、SHX-2、SHX-3、SHX-6、SHX-7、XJ-2和91年分离的HuB-1均属于Ⅶ基因型,该基因型的病毒是九十年代以来引起新城疫发生的主要病原。根据遗传距离和分离年代可将此基因型进一步划分为5个基因亚型,分别是Ⅶa、Ⅶb、Ⅶc、Ⅶd和Ⅶe。此外HuN-1/98、HLJ-4/95和HeN-1P属一个老的基因型Ⅵ,79～85年分离自青海的QH-1、QH-2、QH-4属于一个新的基因型Ⅷ型。可见在我国新城疫的流行是极其复杂的:既有老基因型的危胁(Ⅰ～Ⅵ),又有新基因型(Ⅶ)的流行,更有我国独特Ⅷ和Ⅸ基因型潜伏。更多还原

【Abstract】 We calculated amino acid genetic distance in l-374bp of 68 Newcastle disease virus (NDV) isolates (30 isolates from China) F protein coding region by Jotun Hein method, constructed the phylogenetic tree by the DNAstar software. All analyzed NDV strains were divided into nine genotypes, suggesting that there is a novel genotype of NDV in China, which is phylo-genetically distinct from all other genotypes defined so far. These strains circulated from 1940’s up to now, spanned about 50 years, and were found in China only. We denominated it group IX. Strains from pigeon belonged to group VI, whose prototype virus is a PPMV-1 from pigeon early; maybe this type has some host specificity. NDV strains YN-1P, GX-3, H1, H2, P1, GX-1, GX-2, GS-2, GS-3, SHX-2, SHX-3, SHX-6, SHX-7, XJ-2 and HuB-1 isolated in 1991-1999 are classified into group VII, and related to NDV strains that circulated in chicken in Southern Africa, western Europe and Taiwan in 1990’s. Isolates QH-1, QH-2 and QH-4 from Qinhai province of China and those from Southern Africa consist of a new genotype VIII.All the results showed that NDV circulated in our country are regional to some extent, and also many strains oversea are imported. So it is very complicated especially in regard of different birds species. On the other hand, although NDV is steadily inherited, it is varied actually and may be one reason of ND’s prevailing.更多还原