α-淀粉酶抑制剂微胶囊奶片的制备及其质量评价

【作者】 刘勇；

【导师】 孙庆申； 韩俊；

【作者基本信息】 黑龙江大学 ， 农业硕士（专业学位）， 2022， 硕士

【摘要】 α-淀粉酶抑制剂(α-AI),又被称为“淀粉阻断剂”或“糖类阻滞剂”,是糖苷水解酶抑制剂的一种,广泛存在于动植物体,可以用以治疗高血糖患者的高血糖问题。本研发团队曾从中国东北油豆角籽中提取出α-AI,实验表明它具有优异的降糖功能,但鉴于它对酸性环境的耐受性欠佳,所以本文意在研究一种耐酸性的α-AI微胶囊片剂。方法:利用肠溶性的羧甲基纤维素钠和海藻酸钠为壁材,研制出α-AI微胶囊;以α-AI微胶囊冻干粉为主料、全脂奶粉、脱脂奶粉、白砂糖等为辅助材料,以单因素实验数据结果为基础,根据响应面分析实验对α-AI微胶囊奶片的配方进行优化分析。实验结果如下:白砂糖8%,全脂奶粉50%,微胶囊冻干粉2%,硬脂酸镁3.5%,脱脂奶粉36.5%,该条件下奶片的直径为(1.00±0.05)cm,厚度(0.66±0.05)cm,奶片每片的重量为(0.35±0.08)g,奶片的平均硬度为43.17 N,水分含量(8.43±0.55)%,灰分含量(4.50±0.17)%,奶片通过胃部后到达肠道45 minα-AI全部释放,抑制活性可以达到80%。在加速实验中微胶囊奶片中α-AI的活性可以保留在75%,在25℃及4℃两种保存温度下,α-AI微胶囊奶片在180 d的储藏稳定,4℃的保存温度要优于室温下保藏。本实验制备的α-AI微胶囊奶片进一步丰富了健康食品的种类,为人们餐后的血糖控制提供了一种方便快捷的方法,不仅满足人们对于健康的需求,还满足了人们对于口感和风味的要求。更多还原

【Abstract】 Alpha-amylase inhibitors(α-AI),also known as"starch blockers"or"glycolytic blockers",are a class of glycoside hydrolase inhibitors that are widely found in plants and animals and can be used to treat hyperglycemia in patients with high blood sugar.Our research team has extractedα-AI from northeastern China’s Olea europaea seeds,which has been shown to have excellent hypoglycemic properties,but given its poor tolerance to acidic environments,this paper aims to investigate an acid-tolerantα-AI microencapsulated tablet.Methods:Theα-AI microcapsules were developed by using enteric-soluble sodium carboxymethylcellulose and sodium alginate as wall materials;theα-AI microcapsules lyophilized powder as main ingredients,whole milk powder,skim milk powder and sugar as auxiliary materials,and the formulation ofα-AI microcapsules milk tablets was optimized and analyzed based on response surface analysis experiments with the results of single-factor experimental data.The experimental results were as follows:sugar 8%,whole milk powder 50%,microencapsulated lyophilized powder 2%,magnesium stearate 3.5%,skim milk powder36.5%,the diameter of the milk tablets under this condition was(1.00±0.05)cm,thickness(0.66±0.05)cm,the weight of each tablet was(0.35±0.08)g,the average hardness of the tablets was 43.17 N,moisture content(8.43±0.55)%and ash content(4.50±0.17)%,the milk tablets reached the intestine 45 min after passing through the stomachα-AI was fully released and the inhibitory activity could reach 80%.The activity ofα-AI in microencapsulated milk tablets could be retained at 75%in the accelerated experiment.α-AI microencapsulated milk tablets were stable in storage for 180 d at two storage temperatures of 25℃and 4℃,and the storage temperature of 4℃was better than that at room temperature.Theα-AI microencapsulated milk tablets prepared in this experiment further enriched the variety of health foods and provided a convenient and fast method for people’s postprandial blood glucose control,which not only satisfied people’s demand for health,but also satisfied people’s demand for taste and flavor.更多还原