【作者】 王楠；

【导师】 胡丽；

【作者基本信息】 华中科技大学 ， 口腔医学（专业学位）， 2023， 硕士

【摘要】 目的:随着生活质量的提高和审美需求的增加,越来越多的成年人选择正畸治疗。然而,大量的临床证据表明,许多正畸患者会发生牙周炎症,从而影响正畸治疗的效果或加重牙周状况。因此,明确炎症环境下影响正畸治疗的关键因素,对提高正畸治疗效率至关重要。方法:本研究通过分析公共数据库中的单细胞数据来确定巨噬细胞在正畸治疗中的作用。在正常和牙周炎症环境下建立正畸大鼠模型,通过流式细胞术、组织学染色和micro-CT比较正畸牙移动效率(OTM)、骨代谢水平和巨噬细胞指标。最后,通过分选的CD301b+巨噬细胞回输实验以及巨噬细胞与BMSCs(骨髓间充质干细胞)的体外相互作用验证作用机制。结果:(1)根据现有单细胞测序数据分析,巨噬细胞在正畸牙移动过程中发挥积极作用。(2)mirco-CT检测发现,在轻度炎症环境下,正畸牙齿移动效率较低。(3)流式细胞术和组织学染色显示,当CD301b+巨噬细胞数量增加时,微环境有利于正畸牙移动。(4)将CD301b+巨噬细胞回输后,发现牙周微环境明显改善,牙齿移动距离也增加。(5)体外实验进一步验证了CD301b+巨噬细胞在拉伸力作用下可促进BMSCs成骨分化。结论:炎症微环境下巨噬细胞在正畸治疗中发挥关键作用。其中CD301b+巨噬细胞改善牙周微环境,提高正畸牙移动效率。输注CD301b+巨噬细胞可促进正畸活牙周围的成骨,改善正畸过程中的骨改建,加速正畸牙移动。这些结果表明,CD301b+巨噬细胞可能在正畸治疗的炎症环境中发挥积极作用,并可作为潜在的调控靶点。更多还原

【Abstract】 Background: With improvements in the quality of life and the demand for aesthetics,more and more adults are opting for orthodontic treatment.However,a large body of clinical evidence shows that many orthodontic patients experience periodontal inflammation,which hinders the effect of the treatment or exacerbates the periodontal status.Therefore,determining the key factors that affect orthodontic treatment in an inflammatory environment to improve the efficiency of the treatment is vital.Methods: In this study,single-cell data from public databases were analyzed to determine the role of macrophages in orthodontic treatment.Orthodontic rat models were established in normal and periodontal inflammation environments,and the efficiency of orthodontic tooth movement(OTM),bone metabolism levels,and macrophages indicators were compared by flow cytometry,histological staining,and micro-CT.Finally,the mechanism of action was confirmed by the reinfusion experiment of sorted CD301b+ macrophages and the interaction between macrophages and BMSCs in vitro.Results:(1)According to the analysis of the existing single-cell sequencing data,macrophages play an active role in the process of orthodontic tooth movement.(2)The mirco-CT assay found that orthodontic teeth move less efficiently in a mildly inflammatory environment.(3)Flow cytometry and histological staining showed that the microenvironment favored orthodontic tooth movement when the number of CD301b+ macrophages increased.(4)After reinfusion of CD301b+ macrophages,it was found that the periodontal microenvironment was significantly improved,and the distance of tooth movement was also increased.(5)In vitro experiments further verified that CD301b+ macrophages can promote osteogenesis of BMSCs under tensile force.Conclusions: Taken together,it can be noted that macrophages play a key role in orthodontic treatment in an inflammatory microenvironment.Among them,CD301b+macrophages improved the periodontal microenvironment and the efficiency of orthodontic tooth movement.Infusion of CD301b+ macrophages promoted osteogenesis around orthodontic active teeth,improved bone remodeling during orthodontics,and accelerated orthodontic tooth movement.These findings suggest that CD301b+ macrophages may have an active role during orthodontic treatment in inflammatory settings and can be considered as potential regulatory targets.更多还原