【作者】 王虹；

【导师】 吴琍；

【作者基本信息】 青岛大学 ， 外科学（普外）， 2015， 硕士

【摘要】 目的:检测并分析mi RNA-26a、143、195在家族性乳腺癌、散发性乳腺癌组织及癌旁组织和血清中的表达水平差异,并分析其在家族性乳腺癌患者癌组织和血清中表达变化,以评价其对家族性乳腺癌早期诊断的潜在价值。方法:收集家族性乳腺癌、散发性乳腺癌患者的癌组织、癌旁正常组织及血清标本,并收集健康查体女性血清标本。提取血清及组织标本中的总RNA,进行逆转录和实时荧光定量PCR检测mi RNA-26a、143、195在组织和外周血中的表达情况。用t-检验、Wilcoxon检验及Mann-Whitney检验进行统计学分析。结果:(1)乳腺癌组织中mi RNA-26a、143、195的表达水平均低于癌旁组织(P<0.05),mi R-26a、143在家族性乳腺癌组织中表达水平低于散发性乳腺癌组织(P<0.05)。(2)血清mi RNA-26a、143、195在家族性乳腺癌、散发性乳腺癌中表达水平低于对照组(P<0.05),血清mi RNA-143、195在家族性乳腺癌中的表达水平低于散发性乳腺癌组(P<0.05)。(3)mi RNA-143在组织与血清中的表达水平差异正相关(r=0.545,P<0.05)结论:mi RNA-26a、143、195在乳腺癌组织及血清中表达下调,可能成为乳腺癌筛查和诊断的潜在生物学指标;mi RNA-143在家族性乳腺癌组织及血清中表达明显下调,可能为家族性乳腺癌的生物学指标。更多还原

【Abstract】 Objective To investigate the expression and correlation of micro RNA-26 a,143,195 in tissues and matching sera of patients with sporadic/familial breast cancer(BC)and to find new promising potential biomarkers for BC.Methods Tumor tissues,adjacent non-tumor tissues and matching sera were collected from patients with familial BC and patients with sporadic BC.Normal control serum samples were collected from healthy subjects.Total RNAs were extracted from all these samples and the aberrant expression levels of a panel of 3mi RNAs(mi RNA-26 a,143,195)were quantified by using real-time PCR technique.Wilcoxon test,Mann-Whitney test and t-test were used for statistical analysis.Results mi RNA-26 a,143,195 were significantly down-regulated in the tumor and serum specimens compared with those in normal controls(P < 0.05).mi RNA-26 a,143were under-expressed in familial BC tissues compared with sporadic BC tissues(P<0.05),whereas mi RNA-143,195 were under-expressed in sera of familial BC compared with sporadic BC(P<0.05).Furthermore,a high correlation of mi RNA-143 expression level was found between BC tumors and sera(r=0.545,P<0.05).Conclusions This study highlights the roles of mi RNAs in BC diagnosis.mi RNA-143 could be promising potential blood-based biomarker for familial BC diagnosis.更多还原