OXTR基因多态性与儿童期虐待的交互作用对监狱高危男性服刑人员边缘型人格的影响研究

【作者】 张敏；

【导师】 张宁；

【作者基本信息】 南京师范大学 ， 应用心理学， 2020， 硕士

【摘要】 边缘型人格障碍(borderline personality disorder,BPD)是一种常见的人格障碍,由多种遗传与环境因素引起。已有证据表明儿童期虐待为BPD发生发展的危险环境因素,但对BPD的遗传病理学研究仍处于初步阶段。催产素受体(oxytocin receptor,OXTR)基因对其的影响尚不明确。本研究旨在探究儿童期虐待与OXTR基因多态性及二者的交互作用对边缘型人格的影响。本研究选取765名男性高危服刑人员为研究对象,来自江苏省南京市某监狱的9个监区。根据人格障碍诊断问卷第4版(Personality Diagnostic Questionnaire-4,PDQ-4)中的边缘型分量表得分(BPD得分≥4),将被试划分为边缘型人格组(BP组,n=342)与非边缘型人格组(非BP组,n=441)。运用PDQ-4,儿童期虐待量表简版(CTQ-SF),Barratt冲动性量表第11版(BIS-11)对被试进行问卷调查。采集被试静脉血5ml,提取DNA后,运用多重PCR技术及高通量测序技术对OXTR基因多态性进行基因分型。运用SPSS24.0及Haploview v4.1进行统计分析,结果表明:(1)BP组与非BP组在父母养育方式及父母关系上存在显著差异,BP组的饮酒与吸毒史均高于非BP组,且差异具有显著的统计学意义(p<0.05)。(2)BPD得分与儿童期虐待总分及其各维度均存在显著正相关(p<0.01)。在排除其他类型的虐待影响后,仅情感虐待及情感忽视与BPD得分仍呈显著正相关(p<0.05)。进一步回归分析表明,情感虐待及情感忽视对BPD得分有显著的正向预测作用(R~2=0.106,p<0.001)。(3)BP组与非BP组在OXTR-rs53576、rs237897基因型及等位基因频率上存在显著差异(p<0.05),且BP组的rs53576及rs237897的AA纯合子基因型频率和A等位基因频率显著高于非BP组。(4)OXTR-rs53576与躯体虐待及性虐待之间存在显著的交互作用可预测BPD得分(p<0.05),且rs53576的GG纯合子基因型更易受躯体虐待及性虐待的影响。本研究得出结论,在监狱高危男性服刑人员中,儿童期虐待和OXTR基因变异及二者的交互作用在边缘型人格发生发展中起着重要作用。OXTR基因多态性与儿童期虐待的交互作用验证了不同易感性假说。更多还原

【Abstract】 Borderline personality disorder(BPD)is a common personality disorder caused by a large amount of biological and environmental factors.Accumulating evidence suggests that childhood maltreatment is a risk environmental factor in the development of BPD,but research on the genetic pathology of BPD is still in its early stages,and very little is known about the oxytocin receptor(OXTR)gene.The purpose of this study is to explore the relationship between childhood maltreatment,OXTR gene polymorphisms and their interaction on borderline personality.The study included 765 inmates with high risk from an adult male prison in Nanjing,Jiangsu Province.According to the BPD scores in Personality Diagnostic Questionnaire-4(PDQ-4)(BPD score≥4),participants were divided into borderline personality group(BP group,n=342)and non-borderline personality group(non-BP group,n=441).PDQ-4,Childhood Trauma Questionnaire Short Form(CTQ-SF),Barratt Impulsiveness Scale-11(BIS-11)were administered to assess participants.DNA was extracted from 5 ml of venous blood in 765 high-risk inmates.Genotyping of the OXTR single-nucleotide polymorphisms(SNPs)applied multiple PCR technology and high-throughput sequencing technology.SPSS24.0 and Haploview v4.1 were used for statistical analyses.Main results are as follows:(1)There were significant differences between BP group and non-BP group in parental rearing pattern and parental relationship.The drinking and drug use history in BP group were higher than that in non-BP group.And the differences were statistically significant(p<0.05).(2)There were significant positive correlations between BPD scores and childhood maltreatment total scores and its subtypes(p<0.01).After excluding other types of abuse,only emotional abuse and emotional neglect were still positively correlated with BPD scores(p<0.05).Further regression analyses indicated that emotional abuse and emotional neglect could significantly positive predict BPD scores(R~2=0.106,p<0.001).(3)There were significant differences in the genotype and allele frequencies of rs53576 and rs237897 between the BP and non-BP groups(p<0.05),and the BP group had higher rs53576 and rs237987 AA homozygous and A allele frequencies than that in the non-BP group.(4)The interactions between OXTR-rs53576 and physical abuse and sexual abuse could significantly predict BPD scores(p<0.05),and the rs53576 GG homozygous carriers were more susceptible to physical abuse and sexual abuse.The findings suggest that childhood maltreatment,OXTR gene variations and the interaction between them are important mechanism for the development of BPD.The interaction between OXTR gene polymorphism and childhood maltreatment confirms different susceptibility hypothesis.更多还原