【作者】 丁勇；

【导师】 庞自力；

【作者基本信息】 华中科技大学 ， 泌尿外科， 2019， 硕士

【摘要】 目的:KLHL22分子对膀胱癌的生长、迁移及侵袭的研究。方法:通过阅读文献了解KLHL22分子在人多个疾病的发病机制中所起的作用。综合生物信息数据库查找KLHL22在膀胱癌及正常膀胱组织中的m RNA表达量差异,再通过实时定量PCR和Western blot等实验手段验证其在膀胱癌细胞系与正常尿路粘膜上皮以及临床膀胱癌组织和相应的癌旁组织中m RNA和蛋白质的表达量的差异性。最后检测KLHL22分子对膀胱癌细胞的生长、迁移及侵袭能力的影响,这将为我们深入探索KLHL22的生物学功能奠定实验基础。结果:通过数据库分析发现,KLHL22 m RNA在膀胱癌中表达量较正常膀胱相对较高;实时定量PCR和Western blot也显示KLHL22在膀胱肿瘤组织中m RNA和蛋白质的表达水平明显高于对照组;Ed U细胞增殖实验、划痕实验和transwell侵袭实验显示KLHL22可以促进膀胱癌细胞增殖、迁移和侵袭。结论:KLHL22可以促进膀胱癌细胞的增殖、迁移及侵袭过程。更多还原

【Abstract】 Objective : To explore the biological functions of KLHL22 about the proliferation,migration and invasion of bladder cancer.Methods: Read the literature to understand the role of KLHL22 in the pathogenesis of human diseases;comprehensive bioinformatics database to find the difference in the expression of KLHL22 in bladder cancer and normal bladder tissue,and then through real-time quantitative PCR and Western blot and other experimental methods to verify its differences in bladder cancer cell lines and normal urinary tract mucosal epithelium;clinical bladder cancer tissues and adjacent tissues.Finally,the effects of KLHL22 on the proliferation,migration and invasion of bladder cancer cells were examined,which laid the experimental foundation for studying the biological function of KLHL22.Results: The database analysis showed that KLHL22 was highly expressed in bladder cancer compared with the control group.Real-time quantitative PCR and Western blot also showed that the expression level of KLHL22 in tumor tissues was significantly higher than that in the control group;Ed U,transwell invasion,and scratch experiments showed that KLHL22 could promote Proliferation,invasion and migration of bladder cancer cells.Conclusion: KLHL22 can promote the proliferation,migration and invasion of bladder cancer cells.更多还原