【作者】 杨威；

【导师】 陈有君；

【作者基本信息】 内蒙古农业大学 ， 发酵工程， 2018， 硕士

【摘要】 为了探明环境pH条件对褐环粘盖牛肝菌基因表达的影响,本文测序分析了培养在不同pH环境下的该菌转录组,通过其基因的差异表达推断菌丝体酶活性的变化情况、感受环境pH的机制以及生理代谢的迥异。结果表明,与生长在pH 3.0环境下的菌丝相比,pH 4.0、4.5、5.0、5.5、6.0、7.0与8.0条件下的菌丝,分别有743、870、1177、1512、1628、3135和2902个基因发生了差异表达,这些差异表达基因在基因本体论数据库中分别注释到2029、2368、2733、3125、3168、4626和4728个功能,在京都基因与基因组百科全书中分别定位到76、81、108、121、116、180和177个代谢途径。根据ICL及MS基因的表达量变化情况,发现乙醛酸的累积量与草酸产量随pH的变化趋势一致,呈正相关。根据FUM、CS及MDH基因的表达量变化情况,发现pH6.0条件下乙醛酸、琥珀酸和草酰乙酸累积量较高,7.0、8.0条件下乙醛酸、琥珀酸和苹果酸含量较高,帮助菌丝体适应和调节环境pH。菌丝体质膜上MAPK信号通路、氯离子通道、ABC转运体、G蛋白偶联的信号转导和MFS底物转运体被激活,从而感受环境pH。pH4.5、6.0条件下,脂肪酸代谢、卟啉和叶绿素代谢、类固醇生物合成、牛磺酸、亚牛磺酸代谢、丁酸代谢、丙酮酸代谢和甘氨酸、丝/苏氨酸代谢等诸多代谢途径的活性较高,是菌丝体在该pH条件下生长适宜的重要因素。更多还原

【Abstract】 In order to explore the influence of environmental p H conditions on gene expression of Suillus luteus,sequenced the transcriptome of strain growed in different p H environment.And the result is that,compared with the hyphae grown in p H 3.0,the hyphae at p H 4.0,4.5,5.0,5.5,6.0,7.0 and 8.0 had 743,870,1177,1512,1628,3135 and 2902 genes were differentially expressed respectively,.These differential expression genes were respectively annotated to 2029,2368,2733,3125,3168,4626 and 4728 functions in gene ontology,and set to 76,81,108,121,116,180 and 177 metabolic pathways in the Kyoto Genome and Genome Encyclopedia.Based on the differential expression of its genes,we infer mycelial changes in the activity of enzymes,mechanisms of induction environmental p H,and differences in physiological metabolism.The results showed that the content of glyoxylate was consistent with the oxalic acid production,and presented positive correlation between the two based on the changes in the expression levels of the gene encoding ICL and MS.Higher levels of glyoxylate,succinate,oxaloacetate and malate help the mycelium which are at p H 6.0,7.0,and 8.0 to adapt and regulate the p H of the environment by the expression changes of the gene encoding FUM,CS,ICL and MDH.The MAPK signaling pathway,chloride channel,ABC transporters,G protein-coupled signal transduction and MFS substrate transporter on the plasma membrane of mycelia are activated to sense the p H of the environment.Fatty acid metabolism,porphyrin and chlorophyll metabolism,steroid biosynthesis,taurine and hypotaurine metabolism and butanoate metabolism,pyruvate metabolism,glycine,serine and threonine metabolism,and many metabolic pathways had high activity at p H 4.5 and 6.0,that were important factors for optimal growth of mycelium under p H 4.5 and 6.0 conditions.更多还原