【作者】 王飞；

【导师】 张海涛；

【作者基本信息】 苏州大学 ， 外科学（普通外科）（专业学位）， 2017， 硕士

【摘要】 目的:分析IL-36α在结肠癌患者肿瘤组织和癌旁组织中的表达差异,并且初步探讨IL-36α的表达与结肠癌临床病理因素之间的关系。方法:分别使用免疫组织化学法和实时定量PCR法检测36例结肠癌患者的肿瘤组织与癌旁结肠组织的IL-36α和IL-36αmRNA的表达差异;初步探讨结肠肿瘤组织IL-36αmRNA的表达水平与患者的年龄、性别、肿瘤大小、肿瘤细胞分化程度、淋巴结转移和TNM分期等临床病理特征之间的关系。结果:1、免疫组化结果显示:本研究组结肠癌患者癌旁组织中IL-36α的阳性表达率高于结肠癌肿瘤组织。差异有统计学意义(P<0.05)2、RT-qPCR检测结果显示:本研究组结肠癌旁组织中IL-36αmRNA的表达量高于结肠癌组织。差异有统计学意义(P<0.05)3、结肠癌肿瘤组织中,IL-36αmRNA表达水平与结肠癌患者的性别、年龄、肿瘤直径、肿瘤分化程度(高中低分化)均无明显相关性(P>0.05)。淋巴结是否转移和TNM分期与IL-36αmRNA的表达相关,无淋巴结转移比有淋巴结转移IL-36αmRNA表达水平高;TNM分期越早,IL-36αmRNA表达越高。差异有统计学意义(P<0.05)。结论:癌旁结肠组织中IL-36α表达水平比结肠癌肿瘤组织中高;结肠癌肿瘤组织中IL-36α的表达水平与淋巴结是否转移相关,无淋巴结转移者IL-36α表达水平较有淋巴结转移者高;与TNM分期相关,TNM分期越早,IL-36α表达水平越高,与性别、年龄、肿瘤直径、肿瘤分化程度无明显相关性。更多还原

【Abstract】 ObjectiveAnalysis on the difference of expression of IL-36 in colonic cancer and paracancerous tissues,and to explore the clinical features and expression of IL-36α related colon cancerMethodsImmunohistochemistry and RT-qPCR was performed to investigate the expression of IL-36α and IL-36αmRNA in 36 cases of colon cancer tissue and paracancerous tissues.Preliminary disscuss on the relationship between expression level of IL-36α m RNA in patients with colon cancer and clinical pathological features.Results1.Test results by Immunohistochemistry method showed that positive expression rate of paracancerous colon tissues was significantly higher than that of colon cancer tissue,75.0% and 41.7%,respectively,the difference was statistically significant(P<0.05).2.The results of RT-qPCR showed that the expression of IL-36α mRNA was higher than that of colon carcinoma in the paracancerous colon tissues,respectively,the difference was statistically significant(P<0.05).3.In the colon tissue IL-36α mRNA’s expression level had no relationship with gender,age,tumor diameter,case classification(including differentiation).Lymph node metastasis was correlated with the expression of IL-36α mRNA.The lymph node metastasis rate is higher than that without lymph node metastasis.TNM staging was correlated with the expression of IL-36α mRNA,the earlier the TNM stage,the higher the expression of IL-36α mRNA,respectively,the difference was significant(P < 0.05).ConclusionIL-36α present was high expression in paracancerous colon tissues;low expression in colon cancer tissue which prompt its contribution to the process in colon cancer.The expression level of IL-36α was correlated with the Lymph node metastasis and stage ofTNM,and was not correlated with gender,age,tumor diameter and tumor differentiation.IL-36α’s expression level with the lymph node metastasis rate is higher than that without lymph node metastasis.TNM staging was correlated with the expression of IL-36α,the earlier the TNM stage,the higher the expression of IL-36α.更多还原