【作者】 闫文涛；

【导师】 陆秀君；

【作者基本信息】 沈阳农业大学 ， 风景园林学， 2017， 硕士

【摘要】 本试验以蒙古栎嫩枝为试材,剪取当年生枝条中上部做插穗,采用单一激素ABT1号、NAA、IBA以及混合激素即IBA+NAA=1:1（V:V）4种生长调节剂,分别采取200 mg·L^-1、300mg·L^-1、500mg·L^-1、1000mg·L^-14个浓度梯度,其中低浓度采用浸泡处理2h、4h和6h,高浓度快速浸蘸10s和30 s,以清水作对照,研究不同种类及浓度的外源植物生长调节剂对蒙古栎扦插繁殖的影响。采用高效液相色谱法对其生根过程中内源吲哚乙酸（IAA）、脱落酸（ABA）、玉米素（ZT）、赤霉素（GA₃）4种激素的动态变化进行测定;同时,测定生根过程中插穗内部营养物质（可溶性糖、淀粉、蛋白、总氮、C/N值）的变化,并对生根过程中插穗的基部进行形态学及解剖学观察,探究蒙古栎嫩枝扦插的生根机理。主要研究结果如下:1.外源生长促进剂均可有效的促进蒙古栎嫩枝愈伤组织的发育,其中IBA1000 mg·L^-1和IBA+NAA1000 mg·L^-1处理10 s的愈伤组织发育较好,愈伤组织重量可分别达到0.5 g和0.52 g,但均不能有效促进生根,生根率仅为3.3%。2.生根过程中,插穗内源激素IAA和ZT可以促进愈伤组织的发育,进而促进不定根的形成,ABA抑制不定根的生成;较高水平的GA₃对扦插生根起阻碍作用。IAA/ABA和ZT/ABA比值与愈伤组织的发育呈正相关,GA₃/ABA比值与愈伤组织的发育呈负相关。3.在生根过程中,插穗内可溶性糖含量的变化趋势为下降-上升-下降;淀粉和可溶性蛋白含量呈现缓慢积累,然后在不定根表达期迅速被消耗,在不定根伸长阶段呈现上升趋势;总氮含量在扦插前期显著升高,之后降低并趋于平稳,在不定根伸长期缓慢积累;C/N比值愈高,愈有利于生根。4.蒙古栎扦插生根过程中,根原基细胞大约出现在扦插后15-20 d;扦插后40 d不定根开始突破表皮,根原基由皮部或愈伤组织伸出,属于混合生根类型;在60 d左右成活的插穗均形成完整的根系。更多还原

【Abstract】 In this experiment,the young shoots of Quercus mongolica were used as test materials,Shearing born this year in the middle upper branches as cuttings,Using a single hormone ABT 1.NAA,IB A and the IBA+NAA mixed hormones=1:1（V:V）,four kinds of plant growth regulator respectively take 200 mg·L^-1,300 mg·L^-1,500 mg·L^-1 and 1000 mg·L^-1 a concentration gradient,The low concentration was immersed for 2 h,4 h and 6 h and the high concentration was quick diped for 10 s and 30 s,both compared with water,To explore the influence of different kinds and concentrations of exogenous plant growth regulator on cutting propagation of Mongolian oak.By using the method of high performance liquid chromatography（HPLC）to determine the dynamic changes of endogenous indole acetic acid（IAA）、abscisic acid（ABA）、zeatin（ZT）and gibberellic acid（GA₃）;At the same time,the changes of nutrient（soluble sugar,starch,protein,total nitrogen.C/N）were measured during rooting of cuttings,And morphological and anatomical observation of the basal part of cuttings during rooting,exploring the Rooting Mechanism of Softwood Cuttings of Quercus mongolica.The main results are as follows:1.The exogenous growth enhancers could effectively promote the development of callus of Quercus mongolica.The callus development of IBA1000 mg-L^-1 and IBA + NAA1000 mg·L^-1 for 10 s was better,and the callus weight could reach 0.5 g and 0.52 g,but can not effectively promote rooting,rooting rate was only 3.3%.2.In the process of rooting,endogenous hormones IAA and ZT could promote the development of callus and promote the formation of adventitious roots.ABA inhibited the formation of adventitious roots.The higher level of GA₃ could inhibit the rooting of cuttings.The ratio of IAA/ABA and ZT/ABA was positively correlated with the development of callus,and the ratio of GA₃/ABA was negatively correlated with the development of callus.3.In the process of rooting.the change trend of soluble sugar content in the cuttings was decreased-rising and decreasing;the contents of starch and soluble protein were slowly accumulated and then consumed rapidly in the adventitious root expression stage,and increased in the adventitious root elongation stage.In the early cuttings increased significantly,and then decreased and stabilized in the adventitious root elongation of the slow accumulation;C/N ratio is higher,the more conducive to rooting.4.In the process of rooting of Mongolian oak,the root primordial cells appeared about 15-20 days after cutting.and the adventitious roots began to break through the epidermis at 40 days after cutting.The root primordia originated from the skin or callus and belonged to the mixed rooting type.The surviving cuttings form a complete root system.更多还原