【作者】 吴丹；

【导师】 马楠；

【作者基本信息】 苏州大学 ， 分析化学， 2016， 硕士

【摘要】 癌症诊疗一体化,是指可以同时检测出病人的疾病状态,并根据诊断结果给出相应的治疗方案,对实现个性化医疗具有重要的意义。理想的癌症诊疗是能够拥有成像和治疗为一体的诊疗模式,在疾病部位能够同时产生明显的成像和治疗效果,并且对正常组织有较小的影响。纳米技术的迅速发展为癌症诊疗带来了新的发展机遇。量子点由于其独特的光电学性质在生物成像方面得到了广泛的应用,同时量子点也可以作为药物载体,与光敏剂分子连接后,将能量传递给光敏剂分子进行光动力学治疗,实现了成像和治疗的诊疗模式。本论文合成了水溶性的量子点,构建了由量子点,光敏剂分子二氢卟吩e6,以及淬灭基团BHQ3所组成的二维分子信标探针。它可以被癌细胞和肿瘤模型中的m RNA特异性的激活,其中成像和治疗模式分别通过量子点与淬灭基团BHQ3,量子点与光敏剂分子二氢卟吩e6之间发生的荧光共振能量转移所产生。相对于传统的光敏剂分子二氢卟吩e6而言,二维分子信标探针拥有显著的成像和治疗效果,实现了癌症的诊疗一体化。更多还原

【Abstract】 Cancer theranostics can simultaneously assess the disease state of individual patients, and according to the diagnostic results can also tailor the therapeutic treatment, so it hopes great significance for personalized medicine. Ideal cancer theranostics should possess the imaging and therapy modalities, and it can generate prominent imaging and high therapeutic efficacy in the disease site, but has small side effects for normal tissues.The rapid development of nanotechnology has brought new opportunities for cancer theranostics. Quantum dots(QDs) because of their unique optical and electronic properties have been widely used in biological imaging, while the QDs can be used as drug carriers, and the photosensitizers can be conjugated onto them, then the QDs will transfer the energy to the photosensitizers for photodynamic therapy to achieve imaging and therapy modalities.In this paper, we synthesis the water-soluble QDs, and construct a two dimensional molecular beacon(TMB) probe with a photosensitizer chlorin e6(Ce6), a QD and a dark quencher BHQ3. The TMB can be specifictly activitated by the tumor mRNA of cancer cell lines and xenografted tumor models, then the imaging and therapy modality are generated by the FRET between the QD and the dark quencher BHQ3, the FRET between the QD and the photosensitizer Ce6. Compare to the traditional photosensitizer Ce6, the TMB probe has appreciable imaging and therapeutic efficacy, which realize the cancer theranostics.更多还原