【作者】 高慧；

【导师】 周文文；

【作者基本信息】 浙江大学 ， 食品科学， 2016， 硕士

【摘要】 从韩国泡菜制品中筛选出一株具有快速解酒效果的厌氧菌,通过形态特征观察、理化性质测定、16S rDNA序列测序对其进行鉴定,测定菌株在15 min,30 min,45 min和60 min时对不同浓度乙醇的降解效果,并对其耐酸耐胆盐活性进行分析,最后进行小鼠动物实验确定其体内的快速解酒效果。研究结果表明,通过形态学及分子生物学鉴定该菌株为海氏肠球菌,命名为Enterococcus hirae Y1。菌株的解酒效果表明,在乙醇浓度10%、20%和40%的条件下,1h乙醇减少量分别为21.30%,23.63%和17.80%,表现快速解酒的特性。并且Yl具有良好的酸和胆盐耐受性,在pH 2.5时菌株活菌数达到106 cfu/mL以上,在胆盐质量浓度为0.2g/100 mL的MRS液体培养基中,4 h后菌株Y1的活菌数达到106 cfu/mL以上,表现出良好的益生菌特性。小鼠解酒实验结果表明海氏肠球菌Y1可以减缓乙醇在胃肠道吸收的速度,降低血液中乙醇的浓度,缓解肝脏的乙醇降解压力。同时可以提高饮酒后SOD、CAT、GSH-Px等抗氧化酶的活性,降低饮酒后MDA、ALT和AST的活性,降低脂质过氧化的风险,减少饮酒引起的体内氧化自由基的形成及其对肝脏和其他组织细胞的损伤。综上,海氏肠球菌Y1具有快速解酒的功能并且具有耐酸耐胆盐的益生菌特性,为以后开发活菌快速解酒制品奠定了良好的基础,具有广阔的市场前景。更多还原

【Abstract】 An anaerobic strain with the potential of preventing drunkenness was isolated from kimchi and identified by morphological observation, determination of physical and chemical properties and 16S rDNA sequencing analysis. The ethanol degradation ability of strains in different ethanol concentrations was determined at 15 min,30 min, 45 min and 60 min respectively and the acid and bile tolerances were analyzed. At last, animal experiments in mice was designed to determine the in vivo effect of rapid hangover. The efficient strain was identified as Enterococcus hirae and named Yl. With ethanol concentration of 10%,20% and 40%, the reduction amounts of ethanol in one hour by Y1 were 21.30%,23.63% 和 17.80%respectively, which showed its fast anti-alcoholism properties. Moreover, the Y1 strain had good acid and bile resistance. It grew normally under the acidic condition of pH 2.5 with viable cells of 106 cfu/mL or more. In MRS broth with the bile salt concentration of 0.2 g/100 mL, viable cells of Y1 reached 106 cfu/mL or more after 4 h. The experiment results in mice showed Enterococcus hirae Y1 can reduce the absorption speed of alcohol in the gastrointestinal tract and lower the blood concentrations of ethanol to ease pressure on the liver degradation. At the same time it can increase the activity of antioxidant enzymes SOD, CAT, GSH-Px, etc and reduce the activity of MDA, ALT and AST after drinking. Therefore it can lower the risk of lipid peroxidation and reduce alcohol-induced excessive formation of reactive oxygen species (ROS), which caused liver and other tissue damage because of the limited antioxidant capacity. In summary, Enterococcus hirae Y1 with the function of rapid anti-alcoholism and probiotic properties of resistance to acid and bile salt laid a good foundation for developing viable anti-alcoholism products with broad market prospects.更多还原