【作者】 王丽娜；

【导师】 曹莹；

【作者基本信息】 河南师范大学 ， 无机化学， 2015， 硕士

【摘要】 石榴酸是一种具有抗癌、抗糖尿病、抗高血脂、减肥、抗动脉粥样硬化等功能的活性分子。叶黄素是一种天然类胡萝卜色素,可保护视力、消除自由基。作为新型营养素,二者共同存在的突出问题在于氧化稳定性差、生物利用度低、活性机制不明。本文构建了几种基于石榴酸的营养运送载体,并对疏水性的叶黄素进行了负载,初步评价了载体的包封率、氧化稳定性、抑菌性。在本论文的第一章中,我们对石榴酸和叶黄素的结构、营养功能以及其氧化稳定性、活性机制等方面进行了简单明了的阐述,然后指出目前石榴酸和叶黄素在研究中存在的突出问题。接着重点讲述了几类营养素运送载体以及它们的合成方法和优点,并且指出利用运送载体负载营养素的优点及相关研究现状,最后据此提出了本论文的研究切入点和创新性。第二章中,我们合成了石榴酸酯化甘油酯,并在此基础上构建了类脂质体。结果表明石榴酸酯化甘油酯可在水中组装成囊泡状的类似脂质体的结构。石榴酸的稳定性大大提高。但是该载体无法实现对叶黄素的包载。第三章中,我们利用卵磷脂/胆固醇为辅助材料,形成了石榴酸和叶黄素的复合脂质体,脂质体的粒径大约在30nm~50nm,呈空心圆形囊泡形状。对石榴酸和叶黄素的包封率分别为16.34%和2.23%,脂质体中的石榴酸和叶黄素的氧化稳定性均大幅提高。抑菌性实验表明,其对大肠杆菌有抑制生长的效果。第四章中,我们合成了石榴酸酯化PEG并构建了类脂质体营养运送载体。电镜结果表明石榴酸-PEG酯类脂质体的粒径大约在110nm,近似圆泡形状。该类脂质体可以实现对叶黄素的负载,包封率最高16.5%。氧化稳定性测试结果表明,类脂质体中的石榴酸和叶黄素修饰氧化稳定性均大幅提高,且对大肠杆菌有抑制生长的效果。第五章中,我们合成了石榴酸酯化淀粉,并构建了固体脂质纳米粒。TEM显示它们的粒径大约在40~170nm,呈实心圆球形状。该种固体纳米粒对叶黄素的包封率偏低,约为0.64%。尽管稳定性得到增强,但是对大肠杆菌的抑制生长的效果不太明显。更多还原

【Abstract】 Punicic acid is an active molecule which contains many outstanding functions, such as anti-carcinogenic, anti-diabetes, anti-hyperlipidemia, anti-obesity and anti-atherosclerotic properties. Lutein is one of natural carotenoid pigment and it can protect vision, eliminate free radical. However, as new types of nutrients, the punicic acid and lutein have the same problems, such as the poor oxidation stability, low bioavailability and active mechanism are unknown.This paper mainly synthesized several punicic acid delivery carriers to load of hydrophobic drug lutein. And we also preliminary evaluated the load rate, stability and anti-bacterial property of the carriers.In the first chapter of this paper, we simply described the structure, nutrition function, oxidation stability and reactive mechanism of punicic acid and lutein. Then we pointed some outstanding problems about punicic acid and lutein that exist in the study. Next, we focused on several popular kinds of nutrient transport carrier, and their synthesis methods and advantages. The paper also pointed out the advantages of using nutrients transport carrier to load nutrient, and their related research situation. Finally, we accordingly put forward the research point and innovative of this paper.In the second chapter, we synthesized the monoglycerides of punicic acid, and on this basis to build the niosome The results show that monoglycerides of punicic acid can be assembled into niosome of vesicle structure in the water, and the oxidation stability has a greatly improve. However, It cannot package lutein well.In the third chapter, we mainly prepared punicic acid- lutein compound liposome, lecithin and cholesterol as the auxiliary materials. The size of liposome is about 30nm~50nm, and the shape is like a hollow circular vesicle. The encapsulation rate are about 16.34% and 2.23%. Oxidation stability test results show that the punicic acid and lutein in the liposome have higher oxidation stability. Anti-bacterial experiment reveals that the product can inhibit the growth of Colibacillus.In the fourth chapter, we prepared the punicic acid-PEG ester. TEM result shows that the size of the product is about 110 nm, and it is the shape of approximate circle. This kind of nosisome can load lutein well, and the highest entrapment rate can be 16.5%. Oxidation stability test results show that the punicic acid and lutein in the niosome have higher oxidation stability. And anti-bacterial experiments show that the product can inhibit the growth of Colibacillus.In the fifth chapter of this paper, we synthesized punicic acid esterified soluble starch, and constructed of the SLN. TEM shows that their sizes are respectively about 40~170nm, and it is the solid sphere shape. This kind of SLN can hardly load lutein, and the entrapment rate is about 0.64%. Although the oxidation stability is enhanced, the product can hardly inhibit the growth of Colibacillus.更多还原