【作者】 吴昊；

【导师】 宁海龙；

【作者基本信息】 东北农业大学 ， 作物遗传育种， 2015， 硕士

【摘要】 本研究定位四向重组自交系群体大豆不同生育期的QTL,为大豆生育期性状QTL定位和分子辅助育种提供基础。以垦丰14(P1)、垦丰15(P2)、垦丰19(P3)和黑农48(P4)为亲本,构建了含160个株系的四向重组自交系为试验材料。在大豆四项杂交群体中的主基因分离分析中可知VE-R1时期,2013KS、2014HS1、2014HS1两个环境不存在主基因效应。四向重组自交系群体的R1-R3,在2013KS、2014HS2环境下只存在一个主基因效应,HS1环境下存在多个主基因效应。四向重组自交系群体的R3-R6,在2014HS1环境下只存在一个主基因效应,HS2环境下存在多个主基因效应,2013KS环境下,不存在主基因效应。四向重组自交系群体的R6-R8,2014HS1、2014HS2和2013KS环境下不存在主基因效应。四向重组自交系群体的生殖生长期性状(R1-R8),在2014HS1、2014HS2环境下只存在一个主基因效应。但KS环境下,不存在主基因效应。四向重组自交系群体的全生育期性状(VE-R8),在2014HS1、2014HS2和2013KS环境下,只存在一个主基因效应。应用SSR标记鉴定个体基因型,利用单标记分析方法,对该群体的VE-R1、R1-R3、R3-R6、R6-R8、生殖生长(R1-R8)及全生育期(VE-R8)进行QTL定位分析。VE-R1:在3个环境中检测到控制大豆VE-R1的45个QTL,有6个QTL在2个环境重复检测到,可认为是稳定QTL。能够稳定缩短VE-R1的等位基因型有5个,能够稳定延长VE-R1的等位基因型有3个。R1-R3:在3个环境中检测到控制大豆R1-R3的25个QTL,在被检测到的QTL中,有1个QTL在3个环境重复检测到,1个QTL在两个环境中检测到,可认为是稳定QTL。这些QTL位点中,能够稳定缩短R1-R3的等位基因型有3个,能够稳定延长VE-R1的等位基因型有2个。R3-R6:在3个环境中检测到控制大豆R3-R6的60个QTL,在被检测到的QTL中,其中有1个QTL在3个环境重复检测,有6个QTL在2个环境重复检测到,可认为是稳定QTL。在这些QTL位点中,能够稳定缩短R3-R6的等位基因型有6个,能够稳定延长R3-R6的等位基因型有4个。R6-R8:在3个环境中检测到控制大豆R6-R8的88个QTL,有15个QTL在两个环境中重复检测到,1个QTL在3个环境中检测到,可认为是稳定QTL。在这些QTL位点中,能够稳定缩短VE-R1的等位基因型有6个,能够稳定延长R6-R8的等位基因型有8个。R1-R8:在3个环境中检测到控制大豆R1-R8的19个QTL,有2个QTL在两个环境中重复检测到,可认为是稳定QTL。在这些QTL位点中,能够稳定缩短VE-R1的等位基因型有1个,能够稳定延长R1-R8的等位基因型有1个。VE-R8:在3个环境中检测到控制大豆VE-R8的17个QTL,有3个QTL在两个环境中重复检测到,可认为是稳定QTL。在这些QTL位点中,能够稳定延长VE-R8的等位基因型有2个。更多还原

【Abstract】 For four-way recombinant inbred lines population, there are four alms in one locus, which come from four parents（kengfeng14, kengfeng15, heinong48, kenfeng19）. They contained 160 recombinant inbred lines as materials.In this study, The study of present research is to develop new methods for scgeregating analysis on major gene for FW-PIL,which would be used to predict the existence of major gene and soybean growth period QTL mapping and breeding.Thought scgeregating analysis on major gene for FW-PIL, these results have been proved:VE-R1 : there are several major genes in KS environment and there is no major gene in the other environment;R1-R3 : there is no major gene in the two environments of HS1 and HS2 amd there are several major genes in KS environment;R3-R6 : there is only one major gene in the two environments of HS1 and HS2 amd there are nol major gene in KS environment;R6-R8: here is no major gene in the three environments of KS, HS1 and HS2;R1-R8: there is only one major gene in the two environments of HS1 and HS2 amd there is no major gene in KS environment;VE-R8: there is only one major gene in the three environments.We used single marker analysis method and SSR discriminate genotype of grows period. The SF-ANOVA identified 52 markers as potentially associated with QTLs for VE-R1 flower at P < 0.05. These showed that the QTL of controling quantitative traits and maker had linkage. The ralleles increased R1-R3 at QTLs identified on B2, A1, B1, C2, D1 a, D2, H, I, J, L, M, N. The beneficial genotype contained Sat₂64（A3A3）, BARCSOYSSR₁0₁444（A3A3）,ARCSOYSSR₁8₁813（A3A3）, Sat₁94（A3A3）和 BARCSOYSSR₀8₀966（A3A3）. But Satt665 has been detected in three places（HS and KS）. Satt245, Satt002, BARCSOYSSR₁0₀066, Satt281 has been detected in two places（HS1 and HS2）. The SF-ANOVA identified 25 markers as potentially associated with QTLs for R3-R6 at P < 0.05. These showed that the QTL of controling quantitative traits and maker had linkage. The ralleles increased R6-R8 at QTLs identified on D1 b, C2, G, L, N, K, E, D2, H, A2, O, J, M. The beneficial genotype contained BARCSOYSSR₀2₀607（A2A2）, Satt688（A1A1）, Satt346（A3A3）, Satt125（A2A2）, Satt557（A1A1）, Satt349（A1A1）, Satt288（A2A2）, BARCSOYSSR₀9₀849（A2A2）和 Sat₀92（A1A1）. But Satt557 has been detected in three places（HS and KS）. Satt125 has been detected in two places（HS1 and HS2）. The SF-ANOVA identified 60 markers as potentially associated with QTLs for Pod—seed filling at P < 0.05. These showed that the QTL of controling quantitative traits and maker had linkage. The ralleles increased Pod—seed filling at QTLs identified on A1, B1, B2, C2, D1 b, D2, E, F, H, I, K, M, N, O. The beneficial genotype contained BARCSOYSSR₁9₀356（ A4A4）,Sat₁95（A4A4）, BARCSOYSSR₀2₀607（A1A1）, BARCSOYSSR₁9₀451（A4A4）, BARCSOYSSR₁9₀466（A4A4）, Satt398（A2A2）, Satt577（A3A3）, Satt125（A2A2）, Satt002（A4A4）, Sat₄05（A3A3）, Satt354（A1A1） Satt284（A4A4）, BARCSOYSSR₀6₀011（A3A3）. But Satt125 has been detected in three places（HS and KS）. The SF-ANOVA identified 88 markers as potentially associated with QTLs for seed R6-R8 at P < 0.05. These showed that the QTL of controling quantitative traits and maker had linkage. The ralleles increased seed filling--maturity at QTLs identified on 18 linkage groups. The beneficial genotype contained Satt273（A1A1）, Satt193（A3A3）. Sat₂64, Satt577, Satt345, BARCSOYSSR₁8₀105 has been detected in two places（HS1 and KS）. The SF-ANOVA identified 19 markers as potentially associated with QTLs for VE-R8 at P < 0.05. These showed that the QTL of controling quantitative traits and maker had linkage. The ralleles increased First flower--maturity at QTLs identified on C2, D2, E, O, D1 b, L, F, B2, N. The beneficial genotype contained Satt577, Satt273, Satt458, BARCSOYSSR₁0₀066.Satt281. Satt557 has been detected in two places（HS2 and KS）. The SF-ANOVA identified 17 markers as potentially associated with QTLs for full period at P < 0.05. These showed that the QTL of controling quantitative traits and maker had linkage. The ralleles increased full period at QTLs identified on A2, C1, C2, E, G. The beneficial genotype contained Satt557（A1A1）, Sat₃51（A1A1）.Sat₁25（A1A1）. But Satt307 has been detected in two places（HS2 and KS）. Satt199, Satt557, Satt125 has been detected in three places（HS and KS）.更多还原