【作者】 贾立娜；

【导师】 谭天伟；

【作者基本信息】 北京化工大学 ， 生物化工， 2006， 硕士

【摘要】 氢气是一种理想的清洁能源,有望在将来成为化石燃料的替代品,以解决日益严重的能源危机和环境问题。味精废水是治理难度很高的一种高浓度有机废水,其治理方法国内已经作了多年的研究,若将废水的处理与生物法制取氢气结合起来,将具有极大的经济和社会效益。论文从利用味精发酵废水培养莱茵衣藻产氢出发,考察了废水中主要物质对莱茵衣藻生长和产氢的影响,并采用味精发酵废水对莱茵衣藻进行培养产氢。通过对莱茵衣藻培养基优化,确定以TAP培养基为莱茵衣藻的生长培养基,碳源为1ml/L冰醋酸,氮源为0.4g/L氯化氨,在该培养基中,莱茵衣藻密度可达6.51×106cell/ml。在TAP培养基中少量添加葡萄糖、谷氨酸和尿素,可提高莱茵衣藻藻细胞密度和叶绿素浓度,葡萄糖、谷氨酸、尿素的最佳添加量分别为0.2g/L、0.3g/L、0.1g/L。硫酸氨可以作为莱茵衣藻的氮源使用,在缺氮的TAP培养基中添加一定的硫酸氨可以使莱茵衣藻生长良好,硫酸氨的最佳添加量为0.5g/L。在TAP培养基中去除硫元素确实对莱茵衣藻产氢有明显的促进作用。最大产氢量为1627μl/bottle,比未去硫的样品提高了约8倍。不同硫元素浓度产氢时,当硫元素浓度为75μmol/L及以下时,产氢效果良好。在去硫培养基中,添加适量的葡萄糖、谷氨酸、尿素对莱茵衣藻产氢也有促进作用,最佳葡萄糖、谷氨酸、尿素的添加量分别为0.2/L、0.3/L、0.1g/L。其中又以葡萄糖对莱茵衣藻产氢促进作用最为明显。为TAP培养基样品产氢量的的2.7倍。各种类型废水在经预处理,用水以一定倍数稀释后,可以用来培养莱茵衣藻。综合废水、离交废水、等电废母液的最适宜稀释倍数均为8倍,COD去除率分别为43.14%、48.3%、57.12%。三种废水中,以废母液培养莱茵衣藻最为理想。以废水为培养液考察莱茵衣藻产氢效果比TAP培养基产氢效果差,综合废水和等电废母液产氢量分别为1.29μl/m1和0.96μl/ml。更多还原

【Abstract】 Hydrogen is an ideal energy source that it would be the substitute of the fossil fuels to solute the energy and environment problem. Monosodium glutamate wastewater is difficult to treat and in a large amount. Therefore, combining the Monosodium glutamate wastewater treating and biohydrogen production will obtain a great of advantages in economy, entironment and the society.In order to produce hydrogen by Chlamydomonas reinhardtii in monosodium glutamate wastewater. The effect of monosodium glutamate wastewater to C. reinhardtii growth and hydrogen production was studied.TAP medium was the best for C. reinhardtii growth. The best carbon source is lml/L acetic acid and nitrogen source is0.4g/L ammonium chloride. The cell density could achieved to6.51×106cell/ml when C. reinhardtii grown in such medium.Adding glucose, glutamic acid or urea to TAP medium can increase C. reinhardtii cell density and chlorophyll content.The best concentration of glucose, glutamic acid and urea are0.2g/L、0.3g/L、0.1g/L respectively. Ammonium Sulphate could be used as nitrogen source and facilitated the growth of C. reinhardtii.The best ammonium sulphate was0.5g/LHigher hydrogen evolution could achieve by Sulfur deprivation in TAP medium (TAP-S). Hydrogen accumulation was1627μl/bottle in6days, which was8times than it in Sulfur repletion TAP medium. The hydrogen evolution was always better when sulphate concentration was lower than75μmol/LAdding glucose, glutamic acid or urea to TAP-S medium can increase the hydrogen production. The best concentration of glucose, glutamic acid and urea are0.2g/L、0.3g/L、0.1g/L respectively. Most important, the glucose promoted hydrogen evolution greatly. It was2.7times than the hydrogen accumulation in TAP-S medium.After pretreatment and dilution, the complex wastewater, ion-exchanged wastewater and isoelectric wastewater could be used for C. reinhardtii incubation. All the wastewater diluted by water in8times is the most appropriate procedure. After C. reinhardtii cultured, COD was changed to43.14%、48.3%、57.12%respectively. What is more, as medium of C. reinhardtii, the isoelectric wastewater was the best of all the three type wastewater. However, hydrogen production in such wastewater was less than it in TAP medium. Hydrogen production was1.29μl/ml and0.96μl/ml in complex wastewater and isoelectric wastewater respectively.更多还原