【作者】 王丹；

【导师】 米春梅；

【作者基本信息】 中南大学 ， 临床医学， 2014， 硕士

【摘要】 目的：研究子痫前期患者和正常孕妇血清及胎盘中ox-LDL、 IL-22的表达,探讨血清及胎盘中ox-LDL、IL-22与子痫前期的关系及二者之间的联系。方法：收集2013年06月-2014年02月在湘雅三医院产科住院的子痫前期患者30例,其中子痫前期轻度15例(轻度组),重度子痫前期15例(重度组)。同期正常妊娠孕妇30例(正常组),分别采用ELISA、免疫组化方法检测血清、胎盘组织中ox-LDL、IL-22的表达水平。结果：(1)血清中ox-LDL、IL-22的水平：子痫前期轻度组和重度组患者血清中ox-LDL水平均明显高于正常组(P<0.05),且重度组明显高于轻度组(P<0.05)；子痫前期轻度组患者血清中IL-22水平较正常组有升高趋势,但差异无统计学意义(P>0.05),而重度组患者血清中IL-22水平明显高于正常组及轻度组(P<0.05)；子痫前期患者血清中ox-LDL与IL-22水平呈明显的正相关(r=0.941,P<0.05)。(2)胎盘中ox-LDL、IL-22的水平：ox-LDL主要在胎盘血管壁内皮和间质沉积,其次是滋养细胞。子痫前期轻度组和重度组患者胎盘中ox-LDL表达水平均明显高于正常组(P<0.05),且重度组明显高于轻度组(P<0.05)；子痫前期轻度组患者胎盘中IL-22水平较正常组有升高趋势,但差异无统计学意义(P>0.05),而重度组患者胎盘中IL-22水平高于正常组及轻度组(P<0.05)；子痫前期患者胎盘中ox-LDIL与IL-22表达水平呈显著的正相关(r=0.754,P<0.05)。结论：(1)子痫前期患者血清及胎盘中ox-LDL表达水平升高且与病情严重程度相关,提示其可能在子痫前期发生、发展中起作用。(2)子痫前期患者血清及胎盘中IL-22表达水平升高,且IL-22与ox-LDL水平呈正相关,提示IL-22的表达升高可能是机体的一种代偿保护作用。更多还原

【Abstract】 Objective:Study the expression of ox-LDL, IL-22in serum and placental of patients with preeclampsia and normal pregnant women, find the relationship between ox-LDL, IL-22in serum and placenta of preeclampsia and the connection of the two.Methods:From June2013to February2014,30women with preeclampsia who delivered in the Department of Obstetries,the Third Xiangya Hospital of Central South University, were recruited in the study, including15women with mild preeclampsia (mild group) and15women with severe preeclampsia (severe group). And30healthy pregnant women were taken as normal group, Enzyme-linked immunosorbent assay(ELISA) was used to measure the serum concentration of ox-LDL, IL-22in these women.Immunohistochemical to study the expression levels of ox-LDL,IL-22in placenta.Results:1) The concentrations of ox-LDL,IL-22in serum:The serum concentrations of ox-LDL in mild group and severe group were significantly higher compared with normal group (P<0.05);and the concentrations of ox-LDL in severe group were significantly higher compared with mild group(P<0.05); The serum concentrations of IL-22in mild group tended to increase compared with normal group, but the difference was not statistically significant (P>0.05); The serum concentrations of IL-22in severe group was significantly higher than the normal group and the mild group (P<0.05); The serum concentrations of ox-LDL and IL-22in preeclampsia were significantly positively correlated (r=0.941, P<0.05).2) The expression of ox-LDL,IL-22in placenta:ox-LDL mainly expressed in vascular endothelial and mesenchymal deposition, followed by trophoblast cells. The expression of ox-LDL in placenta of mild group and severe group were significantly higher than that in normal group (p<0.05),and the expression of ox-LDL in severe group was significantly higher than that of mild group(P<0.05);IL-22is mainly expressed in trophoblast cells and endothelial deposition. The expression of IL-22in placenta of mild group tended to increase compared with normal group, but the difference was not statistically significant (P>0.05);while the expression of IL-22in severe group were significantly higher than that in normal group and mild group(P<0.05); The expression of ox-LDL and IL-22in placenta of preeclampsia were significantly positively correlated (r=0.754, P<0.05).Conclusion:(1)The elevated expression of ox-LDL in serum and placenta of patients with preeclampsia are related to the severity of the disease, suggesting it plays a role in the development of preeclampsia.(2)The expression of IL-22in serum and placenta of patients with preeclampsia was increased, and the levels of IL-22and ox-LDL hava an positive correlation,suggesting that the increased expression of IL-22may be a compensatory protective effect of the body.更多还原