【作者】 王笑康；

【导师】 殷军；

【作者基本信息】 沈阳药科大学 ， 生药学， 2007， 硕士

【摘要】 五味子Schisandra chinensis(Turcz.)Baill.是木兰科(Magnoliaceae)落叶木质藤本植物，习称“北五味子”，其药用部位为干燥成熟果实。《本经》将其列为上品，应用历史悠久。中药标准提取物是指采用现代科学技术，对传统中药材进行提取加工而得到的一种具有相对明确的药效物质基础、严格质量标准的一种中药产品，既可以直接入药，也可作为中药制剂的原料药。标准提取物在国内外植物药市场上占有很大比重。本文从化学成分富集入手，通过含量测定方法学考察、制备工艺研究、化学对照品分离鉴定、各产地药材指纹图谱分析，对五味子标准提取物进行了比较全面的研究。研究主要内容结果如下：1．对五味子的化学成分、药理作用、质量评价以及标准提取物作了简要综述。2．分别对五味子三类化学成分的含量测定方法进行考察，对其差异作了初探。确定了能够真实的反映总含量的测定方法，并进行了方法学考察。3．确定了六种五味子标准提取物的整体制备工艺，富集化学成分，从而保证了提取物质量的一致性。通过不同的制备工艺，得到富集程度不同的提取物，以满足不同的需求。4．应用硅胶柱色谱、硅胶制备薄层色谱、凝胶柱色谱、HPLC制备色谱及重结晶等方法对五味子的化学对照品进行研究。纯化了含量测定用对照品。5．考察了不同产地及采收时间的五味子药材提取物，包括四种标准提取物及一个单体化合物含量的测定，从而对其进行品质评价。6．首次应用HPLC指纹图谱技术对五味子木脂素标准提取物进行研究，建立了提取物的指纹图谱质量控制模式，对不同产地及采收时间的五味子药材提取物进行了全面的质量评价，并且通过聚类分析方法验证了其合理性、正确性。7．对标准提取物的水分、灰分进行测定，同时应用原子吸收分光光度法、比色法对其重金属含量进行测定。更多还原

【Abstract】 Fructus Schisandrae, originated from the dried ripening fruits of Schisandra chinensis (Turcz.) Baill. (Magnoliaceae), which is woody climber, is usually called ’Bei Wu-wei-zi’ in mandarin. S. Chinensis was applied in oriental medicine for a long history and ’Shen Nong’s Herbal Classic’ ranked it into the high grade.Standardized Extracts of Traditional Chinese Medicine refers to an extracted and processed product of Chinese crude drug with relatively definite medical substance and stringent quality specification; it can be applied as a medicine or crude drug in pharmaceutics. At present Standardized Extracts take possession of a large proportion of domestic and overseas botanical medicine market.In this study, a relatively allround research of Standardized Extracts of Schisandra was carried out with commence of enrichment of chemical constituents, including investigation of total count assaying methodology, definition of preparation procedure, isolation and elucidation of chemical reference substance, assaying of different habitat and collecting date and HPLC fingerprint analysis.Main research content and results are as follows:1. In this paper, the chemical composition, pharmacological actions, quality evaluation and Standardized Extracts were briefly reviewed and compiled.2. Total count assaying methodology of three types of chemical constituents were screened and ascertained; these assaying results can mostly reflect the realities of content determination.3. Integrated preparation procedures, parameters and criteria of six Standardized Extracts were definite in order to guarantee the quality consistency; furthermore; the chemical constituents were actually enriched in diverse extent to meet different requirements.4. Chemical reference substances were investigated by means of silica gel column chromatography, preparation TLC, Sephadex column chromatography, HPLC preparation chromatography and recrystallization. The standard reference materials for HPLC assaying and fingerprint were depurated.5. The qualities of materials originated different habitat and collecting date and those four Standardized Extracts were evaluated, including content assaying of one compound.6. The method of HPLC fingerprint spectrum on the studies of Standardized Extracts of Schisandra is applied for the first time; quality control pattern of the extracts with fingerprint was established; full-scale quality evaluation on Standardized Extracts originated different habitat and collecting date was set up. The rationality and correctness authenticated by cluster analysis method.7. Moisture and ash content were determined; the content of heavy metals was also analyzed with the method of atomic absorption spectrophotometry and chromatometry.更多还原