【作者】 韩冬；

【导师】 李铎；

【作者基本信息】 浙江大学 ， 食品工程， 2014， 硕士

【摘要】 本研究目的是将以科学的研究方法,通过动物试验进一步证实耐消化糊精、慢消化淀粉和代糖性甜味剂具有降低血脂水平和血糖的慢性病指标,为有效推广相关概念,促进应用,从而改善和平衡目前人群的膳食结构,进一步降低和改善慢性病的发生。实验通过建立小鼠急性高脂动物模型试验,观察多种碳水化合物及淀粉衍生物对血脂的调控作用,同时,观察其脂代谢相关激素的可能性指数改变。实验选取雌雄各半ICR小鼠80只,按雌雄分别、体重随机分为16组,分别为雌雄空白对照组、雌雄高脂模型对照组、雌雄果糖组、雌雄麦芽糖醇组、雌雄木糖醇组、雌雄抗性糊精组、雌雄麦芽糊精组和雌雄变性淀粉组,每组5只。实验共17天,实验期间,各组动物每天灌胃一次,空白对照组和高脂模型对照组均灌胃0.5%的空白溶剂,其余各组分别灌胃相应的受试样品,灌胃量为0.1g/(10g体重)。连续灌胃16天后,正常对照组除外,其余各组动物全部腹腔注射75%蛋黄乳剂0.5毫升/只(其目的是建立急性高脂血症模型,以检测各种检测产品对改善蛋白的急性高血脂和保护肝功能),禁食不禁水12小时,于采血前2小时再灌胃一次,2小时后摘除眼球取血,处死动物,收集血样,保留肝脏组织,测定相关血液及肝脏生化指标。实验结果显示,本实验表明在小鼠模型中,麦芽糖醇、木糖醇和抗性糊精对于急性高脂血症所引起的高血脂、高血糖具有很好地预防作用。麦芽糖醇、木糖醇和抗性糊精喂养的雌性小鼠相对于模型对照具有更低的肝脏甘油三酯、肝脏总胆固醇、血清甘油三酯和血糖水平(p<0.05)。在雄性小鼠中,麦芽糖醇、木糖醇和抗性糊精,相对于模型对照,对血清甘油三酯和高密度脂蛋白胆固醇也具有很好的改善作用(p<0.05)。总体来说,作为耐消化性碳水化合物的糖醇和抗性糊精,由于其特殊的消化模式以及对小肠内的消化酶的拮抗性,从而降低了小鼠的血糖和血脂的升高,同时加强了对急性高血脂症的代偿性。更多还原

【Abstract】 The purpose of this study is to provide further confirmation that digestion-resistant dextrin, slowly digestible starch and sugar substitute sweeteners can lower blood lipid and glucose levels which are both chronic disease indicators, through a scientific study with animal tests, thereby relevant concepts may be popularized effectively, applications be boosted, current diet of the population be improved and balanced, and incidence of chronic diseases be further reduced.In the established mouse models of acute hyperlipidemia, regulation and control of a variety of carbohydrates and starch derivatives on blood lipid was observed and logged, so was possible changes in indices for metabolism-related hormones.80ICR (Imprinting Control Region) mice,40males and40females, were included in the study. The male and female group was divided into8groups, respectively, which included control group, high hyperlipidemia model group, fructose group, maltitol group, xylitol group, resistant dextrin group, ordinary dextrin group and starch group, with5mice in each group.The study lasted17days, during which the tested agents were administered intragastrically to each mice on a daily basis.0.5%solvent was given to the controls, and the dosage was0.1g/10G for mice of all groups.After16days in succession of intragastric administration, mice in all groups, except for the controls, were intraperitoneally injected with75%egg yolk in a dose of0.5ml/mouse (the purpose of which was to established mouse models with acute hyperlipidemia syndrome, to be used in testing of various acute hyperlipidemia detection products, as well as in protecting hepatic functions. And then, after a12hour water fasting and2hours before removing mice eyes to get blood samples, all mice were administered intragastrically. The mice were sacrificed before collecting blood samples. Liver tissue was taken to be used later in determination of related blood and hepatic biochemical indices.It’s shown in the results that preventive effect upon acute hyperlipidemia and hyperglycemia was observed in mice from maltitol group, xylitol group and resistant dextrin group. Levels of hepatic triglycerides, hepatic total cholesterols, serum triglycerides and blood glucose in female mice from maltitol group, xylitol group and resistant dextrin group were lower compared with their corresponding controls. A very good improvement on serum triglycerides and high density lipoprotein levels was found in male mice from maltitol group, xylitol group, and resistant dextrin group as well.Overall, sugar alcohols and resistant dextrins, as digestion-resistant carbohydrate, due to their special mode of digestion and their resistance to digestive enzymes in small intestine, lowered elevated blood glucose and blood lipid levels in mice, and in the meantime, improved the compensatory effect on acute hyperlipidemia..更多还原