【作者】 王海燕；

【导师】 刘能辉；

【作者基本信息】 中南大学 ， 临床医学， 2013， 硕士

【摘要】 目的：探讨peroxiredoxin I在特发性弱精子症患者精子中的表达情况及其与精浆活性氧的关系。方法：研究对象为2012年9月～2012年12月在中南大学湘雅医院生殖中心就诊的不育男性患者(曾无抗氧化治疗病史)。实验组为26名特发性弱精子症患者,对照组为15名有生育史且精液分析各参数正常的健康男性人群,收集新鲜精液。应用鲁米诺化学发光法检测精浆中活性氧(ROS)水平；采用非连续密度梯度法离心分离精子,用蛋白质印迹技术的方法检测两组男性精子中peroxiredoxin I的表达。结果：1.实验组精浆ROS水平高于对照组,与对照组相比,差异在统计学意义上有显著性(P<0.01),两组精浆ROS水平与前向运动精子活动率呈负相关,差异在统计学意义有显著性(r==-0.777,P<0.01)。2.与对照组相比,peroxiredoxin I在特发性弱精子症患者精子中的表达显著降低,差异在统计学意义上有显著性(P<0.01)。两组精子的peroxiredoxin I含量与精浆ROS水平在统计学意义上呈显著负相关(r=-0.474,P<0.01)。3.两组精子peroxiredoxin I的含量与前向运动精子百分率在统计学意义上呈显著正相关(r==0.779,P<0.01)。结论：1.精子中peroxiredoxin I表达下降可能是引起特发性弱精子症发生的重要环节之一。2.高水平ROS可能是引起特发性弱精子症精子活力下降的主要原因之一。更多还原

【Abstract】 Objective:To explore the expression of peroxiredoxin I in patients with idiopathic asthenospermia and its relationship with seminal reactive oxygen species (ROS).Methods:The objects in this study were selected from the infertile male patients (without antioxidant treatment history) in Xiangya Reproductive Center from September2012to December2012.Fresh semen were selected from the experimental group involved26idiopathic asthenospermia patients,and the control group involved15men with fertility history and normal semen analysis parameters. Luminol chemiluminescence method were applied to detect seminal ROS level;Discontinuous density gradient method were used to centrifugalize sperm;Western blotting were utilized to detect the difference of peroxiredoxin I expression between the control group and experimental group.Results:1.The seminal ROS level in experimental groups is significantly higher than in contral group (P<0.01),showed statistically significant negative relationship between seminal ROS level in both groups and sperm activate rate (r=-0.777, P<0.01)2.Compared with the control group,the peroxiredoxin I expression is significantly lower in the experimental group (P<0.01).The sperm peroxiredoxin I content of the two groups was negatively related with seminal ROS level.3.Showed statistically significant positive relationship between peroxiredoxin I content and human sperm progressive motility (r=0.779, P<0.01)Conclusion:1.The decline of peroxiredoxin I expression may be one of the crucial links to cause idiopathic asthenospermia.2.High level ROS is possible one of the main reasons lead to sperm vitality decline in idiopathic asthenospermia patients.更多还原