【作者】 吴海燕；

【导师】 刘厚君；

【作者基本信息】 华中科技大学 ， 皮肤性病学， 2013， 硕士

【摘要】 目的探讨过敏性紫癜(HSP)患儿血清中基质金属蛋白酶-1（MMP-1）、基质金属蛋白酶-3（MMP-3）及基质金属蛋白酶抑制因子-1（TIMP-1）的水平变化及其临床意义。方法采用酶联免疫吸附法(ELISA)检测40例HSP患儿急性期和恢复期及26例健康儿(对照组)血清中MMP-1,MMP-3和TIMP-1水平。结果①HSP急性期组、恢复期组、正常对照组患儿血清MMP-1,MMP-3和TIMP-1水平分别为62.21±10.20ng/mL、32.79±2.06ng/mL与29.12±2.61ng/ml，1158.0±222.7ng/ml、653.0±28.11ng/mL与575.3±37.21ng/ml，1880.0±304.3ng/ml、950.2±44.69ng/ml、与806.4±77.29ng/mL。急性期组与恢复期组比较差异显著，均有统计学意义（P<0.05）；急性期组与正常对照组比较差异显著，有统计学意义（P<0.05,P<0.05,P<0.01)；恢复期组与正常对照组差异无统计学意义（P>0.05）。②MMP-1,MMP-3和TIMP-1水平与过敏性紫癜活动性成正相关，相关系数分别为0.512，0.257，0.501（P <0.01）。③急性期伴有肾损伤组与急性期无肾损伤组中MMP-1,MMP-3和TIMP-1水平分别为118.6±31.76ng/ml与41.46±1.875ng/ml（P<0.001），2013±773.3ng/ml与833.6±23.92ng/ml（P<0.05），3400±972.1ng/ml与1359±92.53ng/ml，P<0.05,差异均有统计学意义。④急性期伴有肾损伤组患儿血清MMP-1、MMP-3水平与24小时尿蛋白水平成正相关，相关系数分别为0.643，0.470（P<0.05）。结论MMP-1,MMP-3和TIMP-1可作为过敏性紫癜疾病早期活动性指标，其中MMP-1，MMP3和TIMP-1水平可能反映过敏性紫癜肾炎的严重程度。检测HSP患儿血清MMP-1,MMP-3及TIMP-1水平对判定其病情、预后和指导治疗均有一定意义。更多还原

【Abstract】 Objective To investigate the serum level of Matrix Metalloproteinase-1(MMP-1)、Matrix Metalloproteinases-3(MMP-3) and Matrix Metalloproteinases Inhibitor-1(TIMP-1) in children with Henoch-Schonlein Purpura and its clinical significance.Method Utilizing Enzyme-Linked Immuno Sorbent Assay(ELISA) to detect theserum level of MMP-1、MMP-3and TIMP-1in40cases of children with HSP inacute phase and convalescence and26cases of healthy children (control group).Results①The serum levels of MMP-1、MMP-3and TIMP-1in children with HSP inacute phase,convalescence and the control group respectively were62.21±10.20ng/mL、32.79±2.06ng/mL and29.12±2.61ng/ml，1158.0±222.7ng/ml、653.0±28.11ng/mL and575.3±37.21ng/ml，1880.0±304.3ng/ml、950.2±44.69ng/ml and806.4±77.29ng/mL;The differences between acute phase and convalescence were allsignificant (p<0.05); Compared with the control group, the acute phase group showedall significant difference with statistical significance (P <0.05, P <0.05, P <0.01);There were no statistically significant differences between convalescence and controlgroup (P>0.05).②The serum levels of MMP-1、MMP-3and TIMP-1in the childrenin acute phase were positive correlation with activity of HSP; and the correlationcoefficient respectively were0.512,0.257,0.501(P <0.01).③The serum levels ofMMP-1、MMP-3and TIMP-1in the children with HSP in acute phase with kidney injury and with no kidney injury respectively were118.6±31.76ng/ml and41.46±1.875ng/ml(P<0.001),2013±773.3ng/ml and833.6±23.92ng/ml(P<0.05),3400±972.1ng/ml and1359±92.53ng/(P<0.05), There were significant differencesbetween them④The serum levels of MMP-1、MMP-3in the children with HSP inacute phase with kidney injury showed positive correlation with24hours urineprotein levels;and the correlation coefficient respectively were0.643，0.470（P<0.05）.Conclusions MMP-1、MMP-3and TIMP-1may be used as early activity index inchildren with HSP. the serum level of MMP-1and MMP-3may reflect the severity ofkidney injury in children with Henoch Schonlein Purpura Nephritis (HSPN). MMP-1and TIMP-1with allergic purpura activity, was consistent. Detecting the serum levelof MMP-1, MMP-3and TIMP-1in children with HSP to determine itscondition,prognosis and guide therapy has certain clinical significance in determiningpatient’s condition,prognosis and guiding therapy.更多还原