【作者】 李霞；

【导师】 刘朝霞；

【作者基本信息】 南昌大学 ， 妇产科， 2012， 硕士

【摘要】 目的：检测子宫内膜异位症（Endometriosis, EMs）组织中CD68、CD163的阳性表达并分析其与微血管生成的相关性，探讨M2型巨噬细胞在子宫内膜异位症发病机制中的作用。方法：用CD68标记巨噬细胞(CD68+)，CD163标记M2型巨噬细胞(CD163+)和CD34标记微血管密度（microvessel density, MVD），采用免疫组织化学Envision二步法检测35例EMs异位内膜、20例EMs在位内膜及20例正常对照组子宫内膜组织中的CD68、CD163的表达及MVD值，采用SPSS17.0统计软件包对结果进行分析。结果：1. EMs患者的异位内膜组及在位内膜组CD68+、CD163+细胞数、MVD均显著高于对照组（P﹤0.01）；2.盆腔子宫内膜异位症I-II期与III-IV期CD68+、CD163+细胞数、MVD比较无显著性差异（P﹥0.05）；3.实验组和对照组月经周期中分泌期与增生期CD68+、CD163+细胞数、MVD均无显著性差异（P﹥0.05）。4. EMs异位内膜组MVD与CD68+、CD163+细胞数呈正相关（r=0.754，P﹤0.01；r=0.808，P﹤0.01）；5. EMs在位内膜组微血管密度与CD68+、CD163+细胞数呈正相关（r=0.762，P﹤0.01；r=0.829，P﹤0.01）。结论：1. M2型巨噬细胞在EMs发生、发展中起着重要的作用。2.微血管生成是EMs发生、发展的重要环节之一，M2型巨噬细胞与EMs微血管生成密切相关。3.阻断局部微环境中巨噬细胞向M2型分化，可能成为EMs治疗的新途径。更多还原

【Abstract】 Objective：To detect the positive expression of CD68, CD163in women with endometriosisas well as its correlation with microvessel density（MVD）and investigate the role ofM2-polarized Macrophages in the pathogenesis of endometriosis.Methods：We employ the CD68, CD163and CD34to mark macrophages, M2-polarizedmacrophage and MVD respectively. Immunohistochemical method（Envision）wasused to detect the expression of CD68, CD163and MVD in ectopic endometrialspecimens obtained from35patients, eutopic endometrial specimens from20patientswith endometriosis and20normal endometrial tissue samples as control group. Theresults were analyzed by the SPSS17.0software.Results：1. The number of CD68+and CD163+macrophages and the MVD were muchhigher in ectopic and eutopic endometrial groups of endometriosis than those in thenormal control(P﹤0.01);2. The number of CD68+and CD163+macrophages and the MVD had nosignificant difference between the clinical stage of I-II and III-IV of the pelvicendometriotic lesions(P﹥0.05);3. The number of CD68+and CD163+macrophages and the MVD had nosignificant difference between proliferative phase and secretory phase both inexperimental and control group(P﹥0.05);4. The MVD in ectopic endometrial group was positively correlated with thenumber of CD68+and CD163+macrophages (r=0.754, P﹤0.01; r=0.808, P﹤0.01);5. The MVD in eutopic endometrial group of endometriosis was positivelycorrelated with the number of CD68+and CD163+macrophages (r=0.762, P﹤0.01;r=0.829, P﹤0.01).Conclusion：1. The M2-polarized macrophages play an important role in the pathogenesis and progression of endometriosis.2. Microangiogenesis is among the key steps in the pathogenesis anddevelopment of endometriosis, M2-polarized macrophages are closely correlated withthe microangiogenesis in endometriosis.3. Blocking the differentiation of macrophages into the M2subtype in the localmicroenvironment may become the potentially new approach in the therapy ofendometriosis.更多还原