【作者】 莫国辉；

【导师】 彭涛；

【作者基本信息】 广西医科大学 ， 外科学， 2012， 硕士

【摘要】 目的：检测甲胎蛋白基因RS12506899, RS16849384, RS7688274,三位点基因型在广西地区原发性肝细胞癌(hepatocellular carcinoma, HCC)患者中的分布,探讨候选基因型对患者血清甲胎蛋白(α-fetoprotein, AFP)水平以及临床病理生物学行为是否存在影响。方法：选取广西医科大学一附院和广西医科大学肿瘤医院2005年3月～2011年3月期间HCC患者177例。所有病例行肝癌切除手术治疗,提取其肿瘤组织DNA,通过实时荧光聚合酶链式反应(RealTime PCR) Taq-man法进行基因分型。对各组基因型间AFP水平及临床病理特点进行比较。结果：采用卡方检验和方差分析对比不同基因型各组间AFP水平、肿瘤直径、乙肝表面抗原的分布情况,差异无统计学意义。无肝硬化的HCC患者AFP水平高于合并肝硬化的HCC患者(P=0.047)。单因素生存分析结果提示p53抗体阳性程度越高,其无瘤生存时间越短(P=0.010)结论：在本组研究人群中,甲胎蛋白候选基因型与HCC患者的AFP水平及其临床参数不相关。更多还原

【Abstract】 Objective To genotype three locus (rs12506899, rs7688274, rs16849384) at α-fetoprotein (AFP) gene in Guangxi patients with hepatocellular carcinoma (HCC) and to explore its association with serum AFP levels and clinical-pathological factors.Methods177cases of HCC were recruited from the First Affiliated Hospital of Guangxi Medical University and the Tumor Hospital of Guangxi Medical University from Mar2005to Mar2011. All patients recieved liver cancer resection. Extracted DNA form patients’ tumor tissue was subjected to genotyping by real time PCR (Taq-man) assay. Association between genotypes and serum AFP level and clinical-pathological factors were analyzed.Results There was no statistically significant association between AFP genotypes and AFP levels. AFP levels in HCC patients without cirrhosis were higher than those with cirrhosis (P=0.047). Patients with higher scoring of TP53staining in tumor tissues had worse survival than those with smaller scoring (p=010).Conclusion In this population, there was no siginificant association between AFP genotypes and serum AFP level and clinical-pathological factors.更多还原