【作者】 周伟；

【导师】 秦仁义；

【作者基本信息】 华中科技大学 ， 普通外科， 2011， 硕士

【摘要】 目的检测miR-146b-5p在胰腺癌细胞中的表达,探讨其与胰腺癌细胞侵袭转移的关系,为胰腺癌发生发展的研究提供新的思路。方法1应用quantitative Real-time PCR(qRT-PCR)的方法检测miR-146b-5p在6种胰腺癌细胞系中的表达情况。2在MIA PaCa-2细胞系中转染miR-146b-5p mimics后检测miR-146b-5p的表达情况。应用划痕实验、Transwell侵袭实验检测上调miR-146b-5p后,MIA PaCa-2细胞侵袭转移能力的变化。结果和正常胰腺组织相比,miR-146b-5p在6种胰腺癌细胞系中均呈低表达,以MIA PaCa-2细胞系最为明显。在MIA PaCa-2细胞系中,转染miR-146b-5p mimics后,miR-146b-5p表达增高,上调miR-146b-5p后的MIA PaCa-2细胞侵袭转移能力降低。结论miR-146b-5p在胰腺癌细胞中低表达,上调miR-146b-5p后MIA PaCa-2细胞侵袭转移的能力受到抑制,有望成为胰腺癌侵袭转移机制研究新靶点。更多还原

【Abstract】 Objective. The aim of this study is to detect the expression of miR-146b-5p in pancreatic carcinoma cell lines and investigate the relationship between miR-146b-5p and the invasion and migration of pancreatic carcinoma cells. In addition it may provide new ideas for the study of development of pancreatic cancer.Methods1、The expression of miR-146b-5p in 6 pancreatic carcinoma cell lines was examined by quantitative Real-time PCR(qRT-PCR)。2、When MIA PaCa-2 cells have transfected with miR-146b-5p mimics ,the expression of miR-146b-5p in them was examined by qRT-PCR. Meanwhile, we investigated the ability of invasion and migration in MIA PaCa-2 cells by scratch migration assay and transwell invasion assay.ResultsCompared with normal pancreatic tissue, qRT-PCR showed that miR-146b-5p was poorly-expressed in six pancreatic carcinoma cell lines, especially in MIA PaCa-2. The expression level of miR-146b-5p was raised after transfected with miR-146b-5p mimics in MIA PaCa-2 cells, meanwhile the ability of invasion and migration was down-regulated.ConclusionsMiR-146b-5p was poorly-expressed in these pancreatic carcinoma cells. When miR-146b-5p has up-regulated in MIA PaCa-2, it could inhibit pancreatic carcinoma cell invasion and migration .,so that it is expected to become a new target mechanism.更多还原