【作者】 刘畅；

【导师】 周恩相；

【作者基本信息】 中南大学 ， 外科学， 2011， 硕士

【摘要】 目的：分析体外培养的人乳腺癌MCF-7肿瘤细胞系SP (side population)细胞的含量；研究二甲双胍、紫杉醇脂质体、他莫西芬对该细胞系SP细胞的影响,以初步了解上述药物对该细胞系SP细胞的杀伤作用,以及药物之间的协同效应。方法：1、体外培养人乳腺癌细胞系MCF-7至对数生长期；2、以0。3mmo1/L二甲双胍、60μg/L紫杉醇脂质体、10μmol/L他莫西芬分别对其进行单一或联合干预24小时；3、制备上述MCF-7细胞悬液,一组加入Hoechst33342和PI染色,另一组同时加入拮抗剂维拉帕米对照,流式细胞仪分析SP亚群比例。结果：1、SP细胞在人乳腺癌细胞系MCF-7的含量为(3.65±0.42)%：2、二甲双胍组、紫杉醇脂质体组、二甲双胍+紫杉醇脂质体组、他莫西芬组、二甲双胍+他莫西芬组中的SP细胞含量分别为(0.72±0.13)%、(3.45±0.32)%、(0.74±0.16)%、(3.56±0.51)%、(0.87±0.12)%,且它们之间SP细胞含量具有差异显著性(p<0.05)；3、受二甲双胍干预的三组SP细胞含量与其余各组分别比较,差异均具有显著性(p<0.01),三组之间两两比较无统计学差异(p>0.05)；4、空白对照组、紫杉醇脂质体组、他莫西芬组之间两两比较无统计学差异(p>0.05)。结论：1、0.3mmol/L二甲双胍可有效杀灭人乳腺癌MCF-7肿瘤细胞系SP细胞；2、实验浓度下三甲双胍与紫杉醇脂质体或他莫西芬联用并未表现出协同效应。更多还原

【Abstract】 Objective:To identify the percentage of SP (side population) cells of MCF-7 cancer cell line, observe whether the metformin, paclitaxil liposome and tamoxifen can kill SP cells and investigate the existence synergistic effects of drugs above.Methods:The MCF-7 cells were treated with metformin (0.3mmol/L), paclitaxil liposome (60μg/L), tamoxifen (10μmol/L) respectively and combined. After staining with Hoechst33342 and PI (the other groups adding Verapamil as control), the percentages of SP cells were detected in flow cytometry.Results:1. The percentage of SP cells in MCF-7 cancer cell line was (3.65±0.42)%.2. The percentages of SP cells of groups metformin, paclitaxil liposome, metformin+paclitaxil liposome, tamoxifen, metformin+ tamoxifen were (0.72±0.13)%, (3.45±0.32)%, (0.74±0.16)%, (3.56±0.51)%, (0.87±0.12)%. There are significant differences among them.3. We found significant difference between 3 groups treated with metformin and other groups (p<0.01), while there were no evidence of significant differences among these 3 groups (p>0.05).4. We found no significant differences after pairwise comparing control group, group treated with paclitaxil liposome and group treated with tamoxifen (p>0.05).Conclusion:1. Metformin in the concentration of 0.1mmol/L is capable of kill SP cell of MCF-7 cancer cell line effectively.2. Metformin combined with paclitaxil liposome or tamoxifen show no synergistic effects.更多还原