【作者】 彭靖；

【导师】 唐中华；

【作者基本信息】 中南大学 ， 普外科乳腺甲状腺， 2011， 硕士

【摘要】 目的探讨紫杉醇化疗敏感及耐药乳腺癌细胞中相关蛋白的表达差异。方法将病理活检确诊的乳腺癌患者,经紫杉醇二周期新辅助化疗后根据其对化疗的敏感程度划分为敏感组及耐药组,分别解冻、裂解、抽提敏感组及耐药组乳腺癌组织的全细胞蛋白质,采用双向凝胶电泳技术分离敏感组与耐药组蛋白质,建立2-DE图谱。利用图像分析软件比较敏感组及耐药组乳腺癌细胞蛋白质2-DE图谱的差异,应用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术鉴定出乳腺癌细胞敏感组及耐药组的差异蛋白质,建立乳腺癌敏感及耐药差异蛋白质谱。结果在紫杉醇化疗敏感组及耐药组乳腺癌组织蛋白质谱中找到13种差异蛋白质。结论1、本研究初步建立了人乳腺癌细胞紫杉醇敏感及耐药相关蛋白质谱。2、紫杉醇化疗敏感与否和不同乳腺癌细胞蛋白质谱中相关蛋白质的表达差异相关。更多还原

【Abstract】 Objective:To investigate the different expressions of the sensitive and resistant proteins in human breast cancer cells with two cycles of neoadjuvant paclitaxel chemotherapy.Methods:The biopsy confirmed breast cancer tissues, after two cycles of neoadjuvant paclitaxel chemotherapy,are divided into the sensitivity and the resistance. Thawing, cracking, extracting their whole cell proteins respectively, separating the proteins by two-dimensional electrophoresis(2-DE), then we can establish the 2-DE map. Using the image analysis software to distinguish the proteins on the 2-DE map, and the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) to identify the different proteins. So we can establish the breast cancer proteomic map between sensitive and resistant proteins.Results:13 different proteins express in the sensitive and resistant proteins in human breast cancer cells with two cycles of neoadjuvant paclitaxel chemotherapy.Conclusion:1.We established the sensitive and resistant proteins spectrum of human breast cancer with two cycles of neoadjuvant paclitaxel chemotherapy2.The sensitivity of the paclitaxel chemotherapy has strong correlation with the difference of the related proteins,which is in the proteome of the different breast cancer cells.更多还原