【作者】 李伟；

【导师】 彭欣；

【作者基本信息】 山东中医药大学 ， 生物医学工程， 2010， 硕士

【摘要】 目的：探索显微图像技术在中医病证学、药效学研究中的应用。通过观测乙酰苯肼(APH)大鼠模型、肢体缺血再灌注损伤(LIRI)大鼠模型微循环的动态图像变化规律,探讨血虚证的多重性、复合性病理变化特点,探讨四物汤等传统名方补血、调血功效的微循环作用机制,为中医微观辩证学和传统名方药效学提供新的研究方法和实验依据。论文分为3部分：①理论研究；②实验研究；③综合讨论。方法：以“BI-2000医学图像分析系统”动态显微图像技术为主,观测分析四物汤对APH大鼠模型以及LIRI模型肠系膜微循环各指标的影响；并采用血液学检测、足底微循环检测等辅助方法,作为佐证实验。其中,应用BI-2000医学图像分析系统,重点观察APH和LIRI两种模型大鼠肠系膜微循环的变化规律及四物汤的干预作用；应用JI-200激光微循环动态分析仪观察四物汤对血虚大鼠足底微循环的变化；应用F-820Sysmex血液分析仪考察四物汤对血虚大鼠血液常规学指标变化；应用LBY-N6K型全自动血流变仪考察四物汤对APH大鼠血液流变学指标的变化。综合分析各项检测指标和实验结果,探究四物汤对APH血虚证微循环及LIRI模型微循环障碍的作用机制及规律。结果：①应用显微图像技术,能直观形象、连续性地观测血虚证模型的形态学变化,以及四物汤调节微循环的作用。图像学检测数据的总体与临床常用实验检测方法相吻合。②四物汤能改变APH和LIRI两种模型大鼠微血管的血色、红细胞流态,明显提高血液流速(BFVA.BFVV).提高血管网交点数、改善血管管径(DV、DA)的异常状况,且存在明显量效关系,并且能明显提高足底微循环流量。③四物汤能明显降低APH模型大鼠的全血黏度(WBV)、全血还原黏度(WBRV).聚集指数(RAI)、刚性指数(RI)以及电泳指数(EI),提高变形指数(RDI)；并且四物汤对LIRI也存在与APH相同的血流变变化趋势。④四物汤能明显改善APH模型大鼠和LIRI模型的一般体征,减轻LIRI模型的足肿胀率,改善两类模型的微循环状况。结论：①动态显微图像技术以其图像清晰、数据精确、操作便捷等优点,可作为微循环形态学检测的重要方法,并进一步深入探讨。②血虚大鼠肠系膜微循环呈现血虚-瘀血-出血三者并见,互为因果的变化特点,LIRI模型存在严重的微循环障碍。③论文从多个角度和指标分析了四物汤对APH以及LIRI模型的影响作用,四物汤具有补血-活血-止血的综合功效,对APH和LIRI两种模型微循环具有改善作用。更多还原

【Abstract】 Objective:Explored micro-image technology used in studying on diseases and syndromes of traditional Chinese medicine and pharmacodynamics.Through observing acetylphenylhydrazine(APH) blood-deficient rat model and limb ischemia-reperfusion injury (LIRI) rat model of microcirculation changes on the dynamic images in order to discuss characteristics of the multiplicity of blood deficiency and complex nature of compound pathological changes in blood deficiency of Si wu tang. Discussed Si wu tang and other traditional ones can enrich the blood and regulate the blood by studying mechanism of microcirculation.Provided a new pharmacodynamic research methods and experimental basis for studying Chinese medicine micro-dialectically and pharmacodynatics of original recipe of Chinese traditional medicine.Paper was divided into three parts:①theoretical research;②experiment study;③integrated discussion.Methods:Used BI-2000 Medical image analysis system as the main method to observe and analyse the affecting indicators of APH rats as well as LIRI model remedyed by Si wu tang. Employed hematology testing and foot microcirculation detection aids,as the evidence.Besides,applicated BI-2000 medical image analysis system,focusing on observing evolution and intervention on mesenteric microcirculation of APH rats and LIRI by Siwutang.Used Laser microcirculation dynamic analyzer JI-200 to observe influence of microcirculation in rat feet caused by Siwutang;Used hematology analyzer F-820 Sysmex to study blood deficienct rats with normal blood indexes change caused by Siwutang;Used automatic hemorheology analyser LBY-N6K to study hemorheology in APH rats caused by Siwutang.Analysis by synthesis is adopted to analyze experimental results and measurement indicators. To explore mechanism of action and rule about APH and LIRI models caused by Si wu tang.Results:①Used micro-image technology to observe blood-deficient models and microcirculation regulated by Si wu tang directly and continuously perceived through the senses.The overall image test data coincided with detecting methods of clinical test which was routinely used.②Si wu tang could change capillary redness of the skin,red blood cell flow pattern of the APH and LIRI rats,and markedly increased blood flow (BFVA、BFVV),improved the vascular network to pay points,improved the abnormal situation of blood vessel diameter(DV、DA),and there was clear dose-response relationship between them.It could also improve the flow of microcirculation in the feet significantly.③Si wu tang could reduce the whole blood viscosity(WBV),the whole blood reduced viscosity (WBRV),aggregation index(RAI),rigidity index (RI),as well as electrophoresis Index(EI),improve the deformation index(RDI) of APH model rats significantly;and Si wu tang LIRI right there with the same blood flow changes APH trends.④Si wu tang could improve the APH and LIRI model rats general symptoms,reducing feet swelling rate significantly. Microcirculation status of the APH and LIRI model rats both had improved.Conclusion:①ynamic micro-imaging technology can be used as an important morphological microcirculation means of detection for its image clarity,data accuracy,easy to handle,etc.And it is worth being studtied furtherly.②Blood deficiency of mesenteric microcirculation in rat showed characteristics which were deficiency of blood-blood stasis-hemorrhage all three could be seen.They were reciprocally cause and effect.LIRI model had serious microcirculation obstacle.③Papers analysed influence about APH and LIRI models caused by Siwutang from multiple angles and indicators,Si wu tang has a new combination with significant benefits which could enrich the blood-invigorate the circulation of blood-Stop bleeding.Microcirculation of APH and LIRI models were improved by Si wu tang.更多还原