【作者】 潘荞；

【导师】 邓治邦；

【作者基本信息】 湖南农业大学 ， 预防兽医学， 2010， 硕士

【摘要】 本文为研究当前日益复杂的病毒与细菌混合感染的致病机理和病毒间的相互作用,提供鉴别诊断及早期诊断病理学依据,降低因混合感染引起的经济损失。本实验通过对昆明小鼠人工腹腔接种猪圆环病毒2型和副猪嗜血杆菌,观察其系统的病理学变化,为建立昆明小鼠感染猪圆环病毒和副猪嗜血杆菌的病理模型奠定了基础。筛选副猪嗜血杆菌的最佳培养基后进行动物实验,成功感染昆明小鼠,并运用Bliss法得出其半数致死量为3.4291×109CFU。对昆明小鼠注射不同剂量的PCV-2病毒液,并设置一组空白对照。发现小鼠在注射病毒期间并没有明显的临床症状,但是将小鼠处死却可以看到较明显的规律性的剖检变化和病理组织学变化,得出了PCV-2对昆明小鼠的最低感染量为TCID50是106 PCV-2病毒液的100倍稀释液。发现其特征性变化为肺出血、脾肿大、淋巴结肿胀、肝局灶性坏死,以及组织学上的免疫器官的淋巴细胞、巨噬细胞的凋亡或坏死。对昆明小鼠注射PCV-2病毒液,在7天后再次对小鼠注射不同剂量的副猪嗜血杆菌,发现随着细菌剂量的增加,其死亡率增加。而随之产生的病理变化也呈现出较规律的变化,HPS/PCV2混合感染组则除了出现HPS单独感染的病理变化外,还出现了PCV-2单独感染所表现的淋巴细胞严重损伤,从而显著加重小鼠的病理变化,说明两种病原体混合感染具有明显的协同致病作用。且死亡小鼠情况较存活的小鼠严重,病程较长的小鼠情况较严重。更多还原

【Abstract】 To study the pathogenesis of coinfection and interaction between virus and bacteria,offer accurate method for differential diagnosis and early diagnosis,cut down economy missing caused by mixed infection.The experiments observed the pathological changes of KM mice in the system by intraperitoneal inoculation of porcine circovirus 2 and Haemophilus parasuis. This is for the the basis of establishes the pathological model of KM mice infected with porcine circovirus and Haemophilus parasuis.Screening the best medium of Haemophilus parasuis for animal experiments, successfully infected KM mice, and the LD50 was 3.4291×109 CFU by Bliss method.The KM mice were injected with different doses of PCV-2 virus solution, and set a blank control. Found that the mice injected with the virus have no significant clinical symptoms. We can see obvious and regular of pathological changes after the mice were killed.And the pathological changes of PCV-2 showed the Minimum amount of virus infection is the PCV-2 solution that the TCID50 was 106 diluted 100 times.The characteristic changes are lung hemorrhage, splenomegaly, swollen lymph nodes, liver focal necrosis, and apoptosis or necrosis in lymphocytes, macrophages of immune organs.The mice were injected with PCV-2 virus solution, then injected with different doses of Haemophilus parasuis after 7 days.Found that with the increasing dose of bacteria, their mortality. The resulting pathological changes also showed a more regular changes.HPS/PCV-2.mixed infection group, except for HPS infection alone appears pathological changes, but also appeared PCV-2 alone expressed in lymphocytes serious injury. So that Significantly increased the pathological changes in mice. Described above showed that mixed infection with two pathogens significant synergistic pathogenicity. Dead mice were more serious than mice survived,and Longer course of mice were more serious.更多还原