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ALA-PDT对CNE细胞迁移和侵袭影响研究
Research on the Migratory and Invasive Effects of CNE Line after ALA Photodynamic Therapy
【作者】 张菁；
【作者基本信息】 复旦大学 ， 耳鼻咽喉头颈外科， 2010， 硕士
【摘要】 目的：观察5-氨基酮戊酸(5-ALA)光动力疗法作用于CNE细胞后细胞生长抑制、迁移和侵袭性的改变。方法：通过MTT检测5-ALA光动力治疗(5-ALA-PDT) CNE细胞后,不同参数(5-ALA浓度及激光器能量密度)下的细胞生长抑制率变化。并观察不同参数下5-ALA-PDT后细胞划痕实验及Transwell侵袭小室试验。结果：5-ALA-PDT能明显抑制鼻咽癌高分化CNE细胞株的生长,但仍有部分细胞存活。5-ALA-PDT后,当药物浓度和能量密度达到一定水平时CNE细胞的迁移是被抑制的。并且在药物浓度在饱和量1mM／L时,细胞迁移距离与光照时间成线性负相关。光动力作用后,CNE细胞的侵袭能力也是被抑制的,与5-ALA浓度及激光的能量密度相关。在浓度相同的前提下,细胞侵袭数与照光时间成线性负相关。结论：5-ALA-PDT作用于CNE细胞后,细胞的迁移和侵袭能力是被抑制的,并与能量密度和药物浓度相关。
【Abstract】 Objective:To observe and detect the change of invasion and migration about CNE cell line after 5-ALA Photodynamic therapy.Methods:To explore the relation between the inhibition rate and 5-ALA concentration & energy density of lasers by MTT. And observing scratch wound assay and matrigel artificial basement membrane invasion assay after photodynamic therapy with different photosensitizer concentration and energy density of lasers.Results:The results show photodynamic therapy can inhibit the growth of CNE line, but there is still a little cell surviving. The migration of CNE cell is inhibited with appropriate photosensitizer concentration and energy density of laser after photodynamic therapy. It exhibits a linear relation between cell migration and the energy density, when the photosensitizer concentration is a maximum effective concentration of 1mM/L. The invasion of CNE cell is also inhibited after photodynamic therapy. There is a relationship between the change of invasion and 5-ALA concentration & energy density of lasers. It exhibits a linear relation between cell invasion and the energy density in the same photosensitizer concentration.Conclusion:The migration and invasion of CNE line are both inhibited after photodynamic therapy. The change of migration and invasion is related to 5-ALA concentration & energy density of laser.