【作者】 魏敏；

【导师】 杨永秀；

【作者基本信息】 兰州大学 ， 妇产科学， 2011， 硕士

【摘要】 目的：探讨不同浓度顺铂对人宫颈癌SiHa细胞体外增殖的抑制作用及对TSLC1基因mRNA表达情况的影响。方法：采用四甲基偶氮唑蓝(tetrzolium-based colorimetric assay, MTT)比色法观察不同浓度(5、10、15、20、25ug/ml)的顺铂(DDP)分别作用24h、48h、72h后对SiHa细胞体外增殖的影响,测定吸光度(OD)值,并计算抑制率。采用实时荧光定量RT-PCR (real-time reverse transcription polymerase chain reaction)技术检测不同浓度顺铂处理不同时间点后TSLC1基因mRNA表达水平的变化。结果：1、人宫颈癌SiHa细胞系中存在TSLC1基因的表达；2、顺铂能够以明显的剂量-时间依赖方式显著抑制人宫颈癌SiHa细胞系的体外增殖(P＜0.01)；3、不同浓度的顺铂干预人宫颈癌SiHa细胞系不同时间后,TSLC1 mRNA的表达显著性的升高(P＜0.05),且其升高呈明显的剂量-时间依赖性关系。结论：1、人宫颈癌SiHa细胞系中存在TSLC1基因的表达。2、顺铂可能通过上调TSLC1基因mRNA的表达而显著抑制人宫颈癌SiHa细胞的体外增殖。更多还原

【Abstract】 Objective:To investigate the effects of DDP at different concentrations on the inhibition of human cervical cancer SiHa cell proliferation and the expression of TSLC1 gene.Methods:After treatment at different concentrations of DDP (0-25ug/ml) for 24h,48h,72h. using MTT to determine the effects on SiHa cell proliferation, then measured OD, and calculated the rate of inhibition. The changes of expression of TSLC1 mRNA were detected by RT-PCR.Results:1. There are expressions of TSLC1 gene in human cervical cancer SiHa cell;2. With time of treatment or dose of DDP increasing, the inhibitory effect of DDP on the extracorporeal proliferation of SiHa cell was more evident(P<0.01).3. Intervention in different concentrations of cisplatin in human cervical carcinoma cell line SiHa different time. TSLC1 mRNA significant expression of the Increased (P<0.05), and showed significant measurement-time-dependent increase.Conclusion:1. There are expressions of TSLC1 gene in human cervical cancer SiHa cell;2. DDP could obviously inhibit the extracorporeal proliferation of SiHa cells by up-regulation of TSLC1 mRNA expression.更多还原