【作者】 刘宁；

【导师】 谭睿；

【作者基本信息】 西南交通大学 ， 生物化学与分子生物学， 2009， 硕士

【摘要】 灵芝为多孔菌科真菌赤芝Ganoderma lucidum（Ley-ss.ex Fr）Karst或紫芝Ganoderma sinense Zhao,Xu et Zhang的干燥子实体。大量药理研究表明,灵芝具有调节免疫、抗肿瘤、抗衰老、提高机体耐缺氧能力等活性。但由于生态环境、品种、采集期以及加工技术的不同,导致灵芝药材的品质差异较大,为了避免因质量差异而影响到灵芝药材的进一步开发利用,因此,有必要对灵芝药材的质量控制方法进行研究,为建立全面、系统的灵芝药材质量控制方法提供实验依据。我国地域辽阔,地理和气候条件复杂,灵芝种类繁多,长期以来,同一灵芝药材多基源情况较为普遍,使灵芝的品种十分复杂。由于目前国内消费者购买灵芝的主要途径是中药材市场和药房,故本文研究的灵芝药材为以上两个途径获得,其实验结果旨在为灵芝药材的鉴别和质量控制提供实验依据。本文实验部分包括质量标准、指纹图谱和红外光谱研究三部分:1.质量标准的研究:对不同来源的灵芝药材进行了理化测试,其中包括水分、总灰分、酸不溶性灰分,浸出物及灵芝多糖的含量测定,分别从定性和定量两个方面对样品进行了研究及综合评价。2.指纹图谱的研究:初步建立了灵芝三萜类组分的高效液相指纹图谱。通过选择色谱柱、流动相系统、检测波长、柱温等一些因素,确定了灵芝药材指纹图谱建立的最佳条件。色谱柱:Dikma Diamond-C₁₈（4.6mm×200mm,5μm）;流动相:乙腈-0.1%醋酸,采用梯度洗脱的方法;流速:0.8ml/min;检测波长:254nm;柱温:35℃。从而初步建立了该药材具有专属性、特征性、稳定性、重现性的HPLC指纹图谱。3.红外光谱的研究:采用红外光谱法对灵芝原药材进行研究,在数据分析中运用了聚类分析的方法,为其鉴别提供了实验依据。通过本论文的研究,希望能为控制灵芝药材的质量方法提供可行的参考依据,对市场所流通的灵芝药材加以统一规范,同时为具有规范化、系统性、完整性、实用性的2010版药典提供参考依据,更多还原

【Abstract】 Ganoderma lucidum fungus is the dry fruit body of Ganoderma lucidum （Ley-ss.ex Fr） Karst. or Ganoderma sinense Zhao, Xu et Zhang of Polyporaceae . A large number of pharmacological studies have shown that Ganoderma lucidum has the activity of immune regulation, anti-tumor, anti-aging and improving the hypoxia activity of the body. But it leads to larger differences in the quality of the crude drug because of the ecological environment, species, collection time, processing technique are different. So it is necessary to study the method of controlling the quality of Ganoderma lucidum and provide experimental foundation for controlling the quality of Ganoderma lucidum in comprehensiveness and systematism.Due to the complicated geographical and climatic conditions, the case of multi-origin is so widespread in Ganoderma lucidum’s species that their species are complicated for a long time. Ganoderma lucidum is mainly bought from the Chinese crude drug market and the Chinese herbal pharmacy for the domestic consumers, and so the Chinese herbal samples we used in the experiment were mainly from these two paths. The results provide experimental foundation for the identification and quality control of Ganoderma lucidum.In this paper, the contents of this experiment were divided into three parts, including quality standards, The high-performance liquid chromatographic （HPLC） fingerprints and infrared fingerprint:1.Study on quality standards: The physical and chemical testings of Ganoderma lucidum from different paths were performed, including the determination of moisture, total ash, acid insoluble ash, extractum and polysaccharide content of Ganoderma lucidum. And these samples were studied and evaluated comprehensively from both qualitative and quantitative aspects.2.Study on the fingerprints: HPLC fingerprints analysis method for the triterpenes of Ganoderma lucidum was set up preliminarily. The best chromatographic conditions were optimized including the column, the mobile phase systems, detection wavelength, column temperature, etc. The column was Dikma Diamond-C₁₈ （4.6 mm×200mm,5μm） ; gradient elution with a mixture of two solvents: solvent A, 0.1% acetic acid and solvent B, acetonitrile; the flow rate was 0.8ml·min^-1; the detection wavelength was 254nm and the column temperature was 35℃.The HPLC fingerprint of Ganoderma lucidum was established preliminarily with good specificity, characteristic, stability and reproducibility.3. Study on infrared spectra: Ganoderma lucidum was studied through infrared spectrometer and the cluster analysis method was used in the data analysis, it provides the experimental foundation for the identification.Through this research, we hope to provide a workable standard for the quality control of Ganoderma lucidum and regulate the circulation of Ganoderma lucidum from the Chinese crude drug market. And we also hope to provide a reference foundation for the 2010 edition of Pharmacopoeia which will have the characteristics of standardized, systematic, integrity, pragmatic.更多还原